Loading patent…
AssigneesRoche Diagnostics GmbH, Institute of Protein Research
InventorsSergey Vladimirovich Biryukov, Peter Nikolaevich Simonenko, Vladimir Anatolievich Shirokov, Alexander Sergeyevich Spirin
Estimating the term…
The method of polypeptide synthesis in eukaryotic or porkaryotic cell-free systems based on a modified verion of synthesis in the continuous flow or continuous exchange modes when, in addition to input into the reaction mixture of components maintaining the synthesis and removal from the reaction mixture of low molecular weight components inhibiting the synthesis, the concentration of at least one of the components selected from the group consisting of Mg2+, K+, NTP, polyamines or their combinations determining the productivity of the synthesis is continuously changed within the given range of concentrations, while the concentrations of the other components are maintained constant.
A method for obtaining polypepoides in a cell-free system by which the reaction mixture is prepared with the use of a cell lysate or cell extract, the parameters of the cell-free system and the mode of synthesis are chosen, the type and parameters of at least one porous barrier are determined, the reaction mixture and the feeding solution are placed in the reaction module, and the synthesis is performed, wherein upon the parameters of the process are chosen, the types of the selected components determining the productivity of the synthesis are selected, the upper and lower limits of the range within which the concentrations of the selected components are changed during the synthesis are defined, the additional mixture containing the selected components is formed, the additional mixture is supplied to the reaction mixture or to the feeding solution, the synthesis is performed with changing concentrations of the selected components within the defined ranges while the concentrations of the other components are maintained constant.
Dependencies are inferred from the claim text.