Method of detecting a microbe in a liquid water sample
OwnerStephen C. Edberginventor
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Abstract
The invention relates to a method of determining the presence or absence of a target microbe in a liquid water sample, the method consisting of a) combining a liquid water sample and a powdered medium having at least one nutrient indicator, the nutrient indicator being selected from the group consisting of: orthonitrophenyl-B-D-glucuronide; B-napthalamide-B-D-glucuronide; α-napthol-B-D-glucuronide; and methylumbelliferyl-B-D-glucuronide, to form a mixture; b) incubating the mixture for a time sufficient to produce a detectable color signal indicative of the presence or absence of a target microbe; and c) observing the signal to determine the presence or absence of the target microbe in said sample.
Claims7
A method of determining the presence or absence of a target microbe in a liquid water sample, said method consisting of the steps of:
- a) combining a liquid water sample and a powdered medium having at least one nutrient indicator, said nutrient indicator being selected from the group consisting of:
- orthonitrophenyl-B-D-glucuronide;
- B-napthalamide-B-D-glucuronide;
- α-napthol-B-D-glucuronide;
- and methylumbelliferyl-B-D-glucuronide, to form a mixture;
- b) incubating the mixture for a time sufficient to produce a detectable color signal indicative of the presence or absence of a target microbe;
- c) observing the signal to determine the presence or absence of said target microbe in said sample.
A method of determining the presence or absence of a target microbe in a liquid water sample, said method consisting of the steps of:
- a) combining a liquid water sample and a powder having at least one nutrient indicator, said nutrient indicator being selected from the group consisting of:
- orthonitrophenyl-B-D-glucuronide;
- B-napthalamide-B-D-glucuronide;
- α-napthol-B-D-glucuronide;
- and methylumbelliferyl-B-D-glucuronide, to form a mixture;
- b) incubating the mixture for a time sufficient to produce a detectable color signal indicative of the presence or absence of a target microbe;
- c) observing the signal to determine the presence or absence of said target microbe in said sample.
A method of determining the presence or absence of a target microbe in a liquid water sample, said method comprising the following steps, in order:
- a) combining a liquid water sample and a powder having at least one nutrient indicator, said nutrient indicator being selected from the group consisting of:
- orthonitrophenyl-B-D-glucuronide;
- B-napthalamide-B-D-glucuronide;
- α-napthol-B-D-glucuronide;
- and methylumbelliferyl-B-D-glucuronide, to form a mixture;
- b) incubating the mixture for a time sufficient to produce a detectable color signal indicative of the presence or absence of a target microbe without performing a preliminary target microbe growth step;
- c) observing the signal to determine the presence or absence of said target microbe in said sample.
Dependencies are inferred from the claim text.