Physiologically active substance EEM-S originating in mushrooms, process for producing the same and drugs
AssigneeLife Science Laboratories, Inc.
InventorsTetsuro Ikekawa, Akiko Ikekawa, Fumitake Shimada
Life of this patent
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Abstract
EEM-S obtained by extracting with hot water or a lower alcohol at least one mushroom selected from among Lentinus edodes, Flammulina velutipes, Hypsizygus marmoreus, Pleurotus ostreatus, Pholiota nameko, Grifola fondosa, Volvariella speciosa ver. speciosa, Lyophllum decastes, blanc du pays, Tricholoma matsutake, Ganoderma lucidum and Phellinus yucatensis, treating the obtained extract by the molecular sieve method and thus eliminating low-molecular-weight and high-molecular-weight fractions: and preparations thereof. This EEM-S exerts physiological effects such as anticancer, immunopotentiating, antioxidative, hypotensive and hypoglycemic effects.
Claims20
A biologically active substance EEM-SB obtained by extracting Hypsizygus marmoreus with water, a lower alcohol having 1-4 carbon atoms, or a mixed solvent of these, and treating the extract using a molecular sieve method, the biologically active substance EEM-SB having a molecular weight of 6,000-60,000, a maximum value of ultraviolet absorption in water of 255-260 nm, a maximum infrared absorption wave number of 1560, 1396, and 1032 cm−1 (including an error of ±50 cm−1), a protein content of 20-30%, a carbohydrate content of 22-32%, and an amino acid composition (molar ratio) of aspartic acid;
- 9.0%, glutamic acid;
- 16.1%, glycine;
- 9.1%, alanine;
- 12.5%, valine;
- 5.7%, arginine;
- 5.6%, and ornithine;
- 7.8% (including an error of ±3%);
- and containing glucose as an essential constituent carbohydrate.
A biologically active substance EEM-SE obtained by extracting Flammulina velutipes with water, a lower alcohol having 1-4 carbon atoms, or a mixed solvent of these, and treating the extract using a molecular sieve method, the biologically active substance EEM-SE having a molecular weight of 6,000-60,000, a maximum value of ultraviolet absorption in water of 255-260 nm, a maximum infrared absorption wave number of 3259, 2927, 1578, 1400, and 1043 cm−1 (including an error of ±50 cm−1), a protein content of 8-18%, a carbohydrate content of 5-10%, and an amino acid composition (molar ratio) of aspartic acid;
- 9.1%, glutamic acid;
- 17.7%, glycine;
- 14.8%, alanine;
- 21.9%, valine;
- 11.8%, arginine;
- 6.8%, and omithine;
- 12.6% (including an error of ±3%);
- and containing glucose as an essential constituent carbohydrate.
The process for producing a biologically active substance EEM-S comprising:
- extracting at least one mushroom selected from Lentinus edodes, Flammulina velutipes, Hypsizygus marmoreus, Pleurotus ostreatus, Pholiota nameko, Grifola fondosa, Volvariella speciosa ver. speciosa, Lyophllum decastes, Blanc du pays, Tricholoma matsutake, Ganoderma lucidum, and Phellinus yucatensis (Phellinus linteus), with water, a hydrophilic solvent, or a mixed solvent of these, and treating the extract using a molecular sieve method to collect a fraction having a molecular weight of 6,000-60,000 and a maximum, value of ultraviolet absorption in water of 255-260 nm, wherein the molecular sieve method uses a molecular sieve membrane (flat membrane), a hollow filter membrane, a permeable membrane, or molecular chromatography.
Dependencies are inferred from the claim text.