Methods for cloning mammals using telophase oocytes
AssigneeTrustee of Tufts College, Ballou Hall
InventorsAlexander Baguisi, Eric W. Overstrom
Life of this patent
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Abstract
The present invention pertains to methods for cloning animals. In particular, the invention includes methods of cloning an animal by combining a genome from an activated donor cell with an activated enucleated oocyte to thereby obtain a nuclear transfer embryo, and impregnating an animal with the nuclear transfer embryo in conditions suitable for gestation of a cloned animal. The invention further relates to methods of chemically enucleating an oocyte having a meiotic spindle apparatus by exposing the oocyte with a compound that destabilizes the meiotic spindle apparatus.
Claims60
A method of cloning a mammal, comprising the steps of:
- a. fusing a somatic activated donor cell and an activated, enucleated oocyte in telophase II and of the same species as the donor cell, to thereby form a nuclear transfer embryo;
- b. impregnating a mammal of the same species as the nuclear transfer embryo with the fused nuclear transfer embryo under conditions suitable for gestation of the cloned mammal;
- and
- c. gestating the embryo in step b., thereby causing the embryo to develop into the cloned mammal.
A method of producing a transgenic mammal, comprising the steps of:
- a. fusing a somatic activated donor cell having a genetically engineered nucleus and an activated, enucleated oocyte in telophase II and of the same species as the donor cell, to thereby form a transgenic nuclear transfer embryo;
- b. impregnating a mammal of the same species as the nuclear transfer embryo with the fused, nuclear transfer embryo under conditions suitable for gestation of the transgenic mammal;
- and
- c. gestating the embryo in step b., thereby causing the embryo to develop into the transgenic mammal.
A method of producing a mammalian nuclear transfer embryo, comprising fusing a somatic activated donor cell and an activated, enucleated oocyte in telophase II and of the same species as the donor cell, to thereby form a nuclear transfer embryo.
A method of cloning a mammalian fetus, comprising the steps of:
- a. fusing a somatic activated donor cell and an activated, enucleated oocyte in telophase II and of the same species as the donor cell, to thereby form a nuclear transfer embryo;
- b. impregnating a mammal of the same species as the nuclear transfer embryo with the fused nuclear transfer embryo under conditions suitable for gestation of the cloned mammalian fetus;
- and
- c. gestating the embryo in step b., thereby causing the embryo to develop into the cloned mammalian fetus.
A method of cloning a non-human mammal, comprising the steps of:
- a. fusing a somatic activated donor cell and an activated, enucleated oocyte in telophase II and of the same species as the donor cell, to thereby form a nuclear transfer embryo;
- b. impregnating a non-human mammal of the same species as the nuclear transfer embryo with the fused nuclear transfer embryo under conditions suitable for gestation of the cloned non-human mammal;
- and
- c. gestating the embryo in step b., thereby causing the embryo to develop into the cloned non-human mammal.
A method of producing a transgenic non-human mammal, comprising the steps of:
- a. fusing a somatic activated donor cell having a genetically engineered nucleus and an activated, enucleated oocyte in telophase II and of the same species as the donor cell, to thereby form a transgenic nuclear transfer embryo;
- b. impregnating a non-human mammal of the same species as the nuclear transfer embryo with the transgenic nuclear transfer embryo under conditions suitable for gestation of the transgenic non-human mammal;
- and
- c. gestating the embryo in step b., thereby causing the embryo to develop into the transgenic non-human mammal.
A method of cloning a non-human mammalian fetus, comprising the steps of:
- a. fusing a somatic activated donor cell and an activated, enucleated oocyte in telophase II and of the same species as the donor cell, to thereby form a nuclear transfer embryo;
- b. impregnating a non-human mammal of the same species as the nuclear transfer embryo with the fused nuclear transfer embryo under conditions suitable for gestation of the cloned non-human mammalian fetus;
- and
- c. gestating the embryo in step b., thereby causing the embryo to develop into the non-human mammalian fetus.
A method of cloning a mammal, comprising the steps of:
- a. fusing a somatic activated donor cell and an activated, enucleated oocyte derived from an oocyte having a first polar body and an extruding second polar body, and of the same species as the donor cell, to thereby form a nuclear transfer embryo;
- b. impregnating a mammal of the same species as the nuclear transfer embryo with the fused nuclear transfer embryo under conditions suitable for gestation of the cloned mammal;
- and
- c. gestating the embryo in step b., thereby causing the embryo to develop into the cloned mammal.
A method of producing a transgenic mammal, comprising the steps of:
- a. fusing a somatic activated donor cell having a genetically engineered nucleus and an activated, enucleated oocyte derived from an oocyte having a first polar body and an extruding second polar body, and of the same species as the donor cell, to thereby form a transgenic nuclear transfer embryo;
- b. impregnating a mammal of the same species as the nuclear transfer embryo with the fused transgenic nuclear transfer embryo under conditions suitable for gestation of the transgenic mammal;
- and
- c. gestating the embryo in step b., thereby causing the embryo to develop into the transgenic mammal.
A method or producing a nuclear transfer embryo, comprising combining a nucleus from a somatic activated donor cell with an activated, enucleated oocyte derived from an oocyte having a first polar body and an extruding second polar body, and of the same species as the donor cell, to thereby form a nuclear transfer embryo.
A method of producing a mammalian fetus, comprising the steps of:
- a. fusing a somatic activated donor cell and an activated, enucleated oocyte derived from an oocyte having a first polar body and an extruding second polar body, and of the same species as the donor cell, to thereby form a nuclear transfer embryo;
- b. impregnating a mammal of the same species as the nuclear transfer embryo with the fused nuclear transfer embryo under conditions suitable for gestation of the mammalian fetus;
- and
- c. gestating the embryo in step b., thereby causing the embryo to develop into the mammalian fetus.
A method of cloning a mammal, comprising the steps of:
- a. injecting contents of a somatic activated donor cell including a nucleus into an activated, enucleated oocyte in telophase H and of the same species as the donor cell, to thereby form a nuclear transfer embryo;
- b. impregnating a mammal of the same species as the nuclear transfer embryo with the fused nuclear transfer embryo under conditions suitable for gestation of the cloned mammal;
- and
- c. gestating the embryo in step b., thereby causing the embryo to develop into the cloned mammal.
A method of producing a transgenic mammal, comprising the steps of:
- a. injecting contents of a somatic activated donor cell including a genetically engineered nucleus into an activated, enucleated oocyte in telophase II and of the same species as the donor cell, to thereby form a transgenic nuclear transfer embryo;
- b. impregnating a mammal of the same species as the nuclear transfer embryo with the fused, nuclear transfer embryo under conditions suitable for gestation of the transgenic mammal;
- and
- c. gestating the embryo in step b., thereby causing the embryo to develop into the transgenic mammal.
A method of producing a mammalian nuclear transfer embryo, comprising injecting contents of a somatic activated donor cell including a nucleus with an activated, enucleated oocyte in telophase II and of the same species as the donor cell, to thereby form a nuclear transfer embryo.
A method of cloning a mammalian fetus, comprising the steps of:
- a. injecting contents of a somatic activated donor cell including a nucleus into an activated, enucleated oocyte in telophase II and of the same species as the donor cell, to thereby form a nuclear transfer embryo;
- b. impregnating a mammal of the same species as the nuclear transfer embryo with the fused nuclear transfer embryo under conditions suitable for gestation of the cloned mammalian fetus;
- and
- c. gestating the embryo in step b., thereby causing the embryo to develop into the cloned mammalian fetus.
A method of cloning a non-human mammal, comprising the steps of:
- a. injecting contents of a somatic activated donor cell including a nucleus into an activated, enucleated oocyte in telophase II and of the same species as the donor cell, to thereby form a nuclear transfer embryo;
- b. impregnating a non-human mammal of the same species as the nuclear transfer embryo with the fused nuclear transfer embryo under conditions suitable for gestation of the cloned non-human mammal;
- and
- c. gestating the embryo in step b., thereby causing the embryo to develop into the cloned non-human mammal.
A method of producing a transgenic non-human mammal, comprising the steps of:
- a. injecting contents of a somatic activated donor cell including a genetically engineered nucleus into an activated, enucleated oocyte in telophase II and of the same species as the donor cell, to thereby form a transgenic nuclear transfer embryo;
- b. impregnating a non-human mammal of the same species as the nuclear transfer embryo with the transgenic nuclear transfer embryo under conditions suitable for gestation of the transgenic non-human mammal;
- and
- c. gestating the embryo in step b., thereby causing the embryo to develop into the transgenic non-human mammal.
A method of cloning a non-human mammalian fetus, comprising the steps of:
- a. injecting contents of a somatic activated donor cell including a nucleus into an activated, enucleated oocyte in telophase II and of the same species as the donor cell, to thereby form a nuclear transfer embryo;
- b. impregnating a non-human mammal of the same species as the nuclear transfer embryo with the fused nuclear transfer embryo under conditions suitable for gestation of the cloned non-human mammalian fetus;
- and
- c. gestating the embryo in step b., thereby causing the embryo to develop into the cloned non-human mammalian fetus.
A method of cloning a mammal, comprising the steps of:
- a. injecting contents of a somatic activated donor cell including a nucleus into an activated, enucleated oocyte derived from an oocyte having a first polar body and an extruding second polar body, and of the same species as the donor cell, to thereby form a nuclear transfer embryo;
- b. impregnating a mammal of the same species as the nuclear transfer embryo with the fused nuclear transfer embryo under conditions suitable for gestation of the cloned mammal;
- and
- c. gestating the embryo in step b., thereby causing the embryo to develop into the cloned mammal.
A method of producing a transgenic mammal, comprising the steps of:
- a. injecting contents of a somatic activated donor cell including a genetically engineered nucleus into an activated, enucleated oocyte derived from an oocyte having a first polar body and an extruding second polar body, and of the same species as the donor cell, to thereby form a transgenic nuclear transfer embryo;
- b. impregnating a mammal of the same species as the nuclear transfer embryo with the fused transgenic nuclear transfer embryo under conditions suitable for gestation of the transgenic mammal;
- and
- c. gestating the embryo in step b., thereby causing the embryo to develop into the transgenic mammal.
A method of producing a mammalian fetus, comprising the steps of:
- a. injecting contents of a somatic activated donor cell including a nucleus into an activated, enucleated oocyte derived from an oocyte having a first polar body and an extruding second polar body, and of the same species as the donor cell, to thereby form a nuclear transfer embryo;
- b. impregnating a mammal of the same species as the nuclear transfer embryo with the fused nuclear transfer embryo under conditions suitable for gestation of the cloned fetus;
- and
- c. gestating the embryo in step b., thereby causing the embryo to develop into the mammalian fetus.
Dependencies are inferred from the claim text.