DNA encoding a plant lipase, transgenic plants and a method for controlling senescence in plants
AssigneeSenesco, Inc.
InventorsJohn E. Thompson, Tzann-Wei Wang, Katalin Hudak, Yuwen Hong
Life of this patent
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Abstract
Regulation of expression of senescence in plants is achieved by integration of a gene or gene fragment encoding senescence-induced lipase into the plant genome in antisense orientation. The carnation and Arabidopsis genes encoding senescence-induced lipase are identified and the nucleotide sequences are used to modify senescence in transgenic plants.
Claims51
An isolated DNA molecule encoding senescence-induced lipase, wherein the DNA molecule has at least 90% sequence similarity to SEQ ID NO:
- 1, or SEQ ID NO:18, and wherein the DNA molecule encodes an amino acid sequence that has senescence-induced lipase activity of SEQ ID NO:2 or SEQ ID NO:19.
An isolated DNA molecule encoding senescence-induced lipase wherein said DNA molecule comprises SEQ ID NO:1.
An isolated DNA molecule encoding senescence-induced lipase wherein said DNA molecule comprises SEQ ID NO:18.
A vector for transformation of plant cells comprising
- (a) an antisense polynucleotide complementary to SEQ ID NO:1, or SEQ D NO:18, and
- (b) regulatory sequences operatively linked to the antisense polynucleotide such that the antisense polynucleotide is expressed in a plant cell into which it is transformed.
An isolated antisense polynucleotide encoding an RNA molecule, wherein said polynucleotide has at least 90% sequence similarity to the complement of SEQ ID NO:
- 1, or SEQ ID NO:
- 18, wherein the polynucleotide inhibits expression of an endogenous senescence-induced lipase gene when expressed in a plant cell.
A vector comprising
- (a) a DNA molecule encoding senescence-induced lipase, wherein the DNA molecule has at least 90% sequence similarity to SEQ ID NO:
- 1 or SEQ ID NO:18;
- and
- (b) regulatory sequences operatively linked to the DNA molecule,
- (c) wherein the DNA molecule encodes an amino acid sequence that has senescence-induced lipase activity of SEQ ID NO:2 or SEQ ID NO:19.
A method for inhibiting expression of an endogenous senescence-induced lipase in a plant, said method comprising
- (1) integrating into a plant genome a vector comprising
- (A) an antisense polynucleotide having at least 90% sequence similarity to the complement of SEQ ID NO:
- 1 or SEQ ID NO:18;
- and
- (B) regulatory sequences operatively linked to the antisense polynucleotide such that the antisense polynucleotide is expressed;
- and
- (2) growing said plant, whereby the antisense polynucleotide is transcribed and binds to an RNA transcript of an endogenous senescence-induced lipase gene, whereby expression of the senescence-induced lipase gene is inhibited.
A method for inhibiting the expression of an endogenous senescence-induced lipase gene or genes in a plant cell, said method comprising
- (1) integrating into a genome of at least one cell of the plant a vector comprising
- (A) an isolated DNA molecule encoding an exogenous senescence-induced lipase, wherein the DNA molecule comprises SEQ ID NO:
- 1 or SEQ ID NO:18, or wherein the DNA molecule has at least 90% sequence similarity to SEQ ID NO:
- 1 or SEQ ID NO:18, and wherein the DNA molecule encodes an amino acid sequence that has senescene-induced lipase activity of SEQ ID NO:
- 2 or SEQ ID NO:
- 19;
- and
- (B) regulatory sequences operatively linked to the DNA molecule such that the DNA molecule is transcribed, and
- (2) growing said plant, wherein the endogenous senescence-induced lipase is co-suppressed.
A method of delaying age-related senescence and environmental stress-related senescence in a plant, said method comprising
- (1) integrating a vector into a plant genome, the vector comprising
- (A) an antisense polynucleotide having at least 90% sequence similarity to the complement of SEQ ID NO:1 or SEQ ID NO:18, and
- (B) regulatory sequences operatively linked to the antisense polynucleotide such that the antisense polynucleotide is expressed;
- and
- (2) growing said plant, whereby the antisense polynucleotide is transcribed and binds to an RNA transcript of an endogenous senescence-induced lipase gene, whereby expression of the senescence-induced lipase gene is inhibited,
- (3) whereby age-related and environment stress-related senescence is delayed.
A plant and progeny thereof, wherein the plant is derived from a cell having inhibited or reduced expression of senescence-induced lipase, wherein said cell is produced by
- (1) integrating into a cell genome a vector comprising
- (A) an antisense polynucleotide having at least 90% sequence similarity to the complement of SEQ ID NO:
- 1 or SEQ ID NO:18;
- and wherein the polynucleotide inhibits expression of an endogenous senescence-induced lipase gene, and
- (B) regulatory sequences operatively linked to the antisense polynucleotide such that the antisense polynucleotide is expressed;
- and
- (2) growing said plant, whereby said antisense polynucleotide is transcribed and binds an RNA transcript of an endogenous senescence induced lipase gene whereby expression of said senescence-induced lipase gene is inhibited;
- and
- (3) wherein the plant and progeny thereof comprise the vector.
A method of inhibiting seed aging, said method comprising
- (1) integrating into a plant genome a vector comprising
- (A) an antisense polynucleotide having at least 90% sequence similarity to the complement SEQ ID NO:
- 1 or SEQ ID NO:18;
- and
- (B) regulatory sequences operatively linked to the antisense polynucleotide;
- and
- (2) growing said plant, whereby said antisense polynucleotide is transcribed and binds to an RNA transcript of an endogenous senescence-induced lipase gene whereby expression of said senescence-induced lipase gene is inhibited,
- (3) whereby seed aging is inhibited.
A method of increasing seed yield from a plant, said method comprising
- (1) integrating into a plant genome a vector comprising
- (A) an antisense polynucleotide having at least 90% sequence similarity to the complement of SEQ ID NO:
- 1 or SEQ ID NO:18;
- and
- (B) regulatory sequences operatively linked to the antisense polynucleotide;
- and
- (2) growing said plant, whereby said antisense polynucleotide is transcribed and binds to an RNA transcript of an endogenous senescence-induced lipase gene whereby expression of said senescence-induced lipase gene is inhibited,
- (3) whereby seed yield is increased.
A vector comprising
- (a) a DNA molecule encoding a senescence-induced lipase, wherein the DNA molecule comprises SEQ ID NO:1 or SEQ ID NO:18;
- and
- (b) regulatory sequences operatively linked to the DNA molecule.
A method for inhibiting expression of an endogenous senescence-induced lipase in a plant, said method comprising
- (1) integrating into a plant genome a vector comprising
- (A) an antisense polynucleotide complementary to a DNA molecule encoding the endogenous senescence-induced lipase, wherein the DNA molecule encoding the endogenous senescence-induced lipase comprises SEQ ID NO:1 or SEQ ID NO:18;
- and
- (B) regulatory sequences operatively linked to the antisense polynucleotide such that the antisense polynucleotide is expressed;
- and
- (2) growing said plant, whereby the antisense polynucleotide is transcribed and binds to an RNA transcript of said DNA molecule, whereby expression of the DNA molecule is inhibited.
A method for inhibiting the expression of an endogenous senescence-induced lipase gene or genes in a plant, said method comprising
- (1) integrating into a genome of at least one cell of the plant a vector comprising
- (A) an isolated DNA molecule encoding exogenous senescence-induced lipase comprising SEQ ID NO:
- 1 or SEQ ID NO:18;
- and
- (B) regulatory sequences operatively linked to the DNA molecule such that the DNA molecule is transcribed, and
- (2) growing said plant, wherein the endogenous senescence-induced lipase is co-suppressed.
A method of altering age-related senescence and environmental stress-related senescence in a plant, said method comprising
- (1) integrating a vector into a plant genome, the vector comprising
- (A) an antisense polynucleotide complementary to SEQ ID NO:1 or SEQ ID NO:18;
- and
- (B) regulatory sequences operatively linked to the antisense polynucleotide such that the antisense polynucleotide is expressed;
- and
- (2) growing said plant, whereby the antisense polynucleotide is transcribed and binds to an RNA transcript of an endogenous senescence-induced lipase gene, whereby expression of the senescence-induced lipase gene is inhibited.
A plant or progeny thereof, wherein the plant is regenerated from a cell having inhibited or reduced expression of senescence-induced lipase, wherein said cell is produced by
- (1) integrating into the cell genome a vector comprising
- (A) an antisense polynucleotide complementary to SEQ ID NO:1 or SEQ ID NO:18;
- and
- (B) regulatory sequences operatively linked to the antisense polynucleotide such that the antisense polynucleotide is expressed;
- and
- (2) growing said plant, whereby said antisense polynucleotide is transcribed and binds an RNA transcript of an endogenous senescence induced lipase gene whereby expression of said senescence-induced lipase gene is inhibited;
- and
- (3) wherein the plant or progeny thereof comprise the vector.
A method of inhibiting seed aging, said method comprising
- (1) integrating into a plant genome a vector comprising
- (A) an antisense polynucleotide complementary to SEQ ID NO:1 or SEQ ID NO:18;
- and
- (B) regulatory sequences operatively linked to the antisense polynucleotide;
- and
- (2) growing said plant, whereby said antisense polynucleotide is transcribed and binds to an RNA transcript of an endogenous senescence-induced lipase gene whereby expression of said senescence-induced lipase gene is inhibited,
- (3) whereby seed aging is inhibited.
A method of increasing seed yield from a plant, said method comprising
- (1) integrating into a plant genome a vector comprising
- (A) an antisense polynucleotide complementary to SEQ ID NO:1 or SEQ ID NO:18;
- and
- (B) regulatory sequences operatively linked to the antisense polynucleotide;
- and
- (2) growing said plant, whereby said antisense polynucleotide is transcribed and binds to an RNA transcript of an endogenous senescence-induced lipase gene whereby expression of said senescence-induced lipase gene is inhibited,
- (3) whereby seed yield is increased.
Dependencies are inferred from the claim text.