Frozen food product
AssigneeGood Humor-Breyers Ice Cream, Division of Conopco, Inc.
InventorsChristopher M Sidebottom, Margaret F Smallwood, Louise J. Byass
Life of this patent
Estimating the term…
Abstract
Anti-freeze protein which can be derived from Lichen, said protein having an apparent molecular weight of from 20 to 28 kDa and having an N-terminal amino acid sequence which shows at least 80% overlap with: A-P-A-V-V-M-G-D-A-E-S-F-G-A-I-A-H-G-G-L and modified versions and isoforms of this protein.
Claims19
An isolated protein with a molecular weight of about 24 kDa and an isoelectric point of about less than 4.5, comprising an N-terminal amino acid sequence which contains up to two conservative amino acid substitutions in SEQ ID NO:
- 1 or 2, wherein the protein displays substantial ice recrystallization inhibitory activity.
An isolated protein produced by the method of:
- (a) preparing a solution extract of Umbilicaria antarctica,
- (b) isolating a fraction of the solution extract that displays substantial ice recrystallization inhibitory activity,
- (c) isolating a sample of proteins with a molecular weight of about 24 kDa and an isoelectric point of less than 4.5 from said fraction of solution extract, and
- (d) isolating a protein with an N-terminal amino acid sequence which contains up to two conservative amino acid substitutions in SEQ ID NO:
- 1 or 2 from said sample of proteins.
An isolated protein with a molecular weight of about 12 kDa and an isoelectric point of about less than 4.5, comprising an N-terminal amino acid sequence comprising SEQ ID NO:
- 1, wherein the protein displays substantial ice recrystallization inhibitory activity.
An isolated protein with a molecular weight of about 12 kDa and an isoelectric point of about less than 4.5, comprising an N-terminal amino acid sequence comprising SEQ ID NO:
- 2, wherein the protein displays substantial ice recrystallization inhibitory activity.
Dependencies are inferred from the claim text.