Phosphodiester-a-GlcNAcase of the lysosomal targeting pathway
AssigneeGenzyme Glycobiology Research Institute, Inc.
InventorWilliam M. Canfield
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Abstract
The present invention provides nucleotide and amino sequences of the lysosomal targeting pathway enzyme phosphodiester α-GlcNAcase, methods of producing and methods of purifying this enzyme.
Claims43
An isolated phosphodiester α-GlcNAcase, encoded by a nucleic acid sequence that hybridizes under stringent conditions with a complementary sequence of the isolated nucleic acid sequence in SEQ ID NO:7, wherein said stringent conditions comprise washing in 0.2×SSC and 0.1% SDS at 65° C.
An isolated phosphodiester α-GlcNAcase, encoded by a nucleic acid sequence that hybridizes under stringent conditions with a complementary sequence of the isolated nucleic acid sequence in SEQ ID NO:18, wherein said stringent conditions comprise washing in 0.2×SSC and 0.1% SDS at 65° C.
An isolated phosphodiester α-GlcNAcase, encoded by a nucleic acid sequence that hybridizes under stringent conditions with a complementary sequence of the isolated nucleic acid sequence in SEQ ID NO:14, wherein said stringent conditions comprise washing in 0.2×SSC and 0.1% SDS at 65° C.
Dependencies are inferred from the claim text.