Multiphoton excitation microscope for biochip fluorescence assay
AssigneeNational Taiwan University
InventorsChen-Yuan Dong, Peter T. C. So, Sunney I. Chan
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Abstract
A multiphoton excitaion microscope for simultaneously detecting differently colored fluorescence materials on biochips includes a multiphoton excitation source, objectives, and a plurality of detection channels. The biochip is hybridized and labeled with fluorescence materials for expressing hybridized biological signals. The multiphoton excitation source is focused to a light spot on the biochip to excite the fluorescence materials bound thereon. After that, the fluorescence emission at different wavelengths from the different fluorescent materials can be detected by the plural detection channels.
Claims16
A multiphoton excitation microscope for investigating expression of optical signals on a biochip wherein an array of probes fabricated on said biochip is hybridized with sample extractions labeled with fluorescence, which comprises:
- a multiphoton excitation source to excite a light beam;
- a beam scanner receiving the light beam transmitted from said multiphoton excitation source;
- a dichroic mirror reflecting the light beam from said scanner;
- an objective focusing the light beam to a light spot for exciting said optical signals;
- said dichroic mirror separating the fluorescent light from said optical signals;
- a plurality of filters for filtering respective wavelengths of fluorescence of the fluorescent light;
- and
- a plurality of detection channels for detecting respective fluorescence simultaneously.
A multiphoton excitation microscope for investigating expression of optical signals on a biochip wherein an array of probes fabricated on said biochip is hybridized with sample extractions labeled with fluorescence, which comprises:
- a multiphoton excitation source to excite a light beam;
- a beam scanner receiving the light beam transmitted from said multiphoton excitation source;
- a beam mirror means for amplifying and paralleling the light beam from said beam scanner;
- a dichroic mirror reflecting the light beam from said beam mirror means;
- an objective focusing the light beam to a light snot for exciting said optical signals;
- said dichroic mirror separating the fluorescent light from said optical signals;
- a plurality of filters for filtering respective wavelengths of fluorescence of the fluorescent light;
- and
- a plurality of detection channels for detecting respective fluorescence simultaneously.
A multiphoton excitation microscope for investigating expression of optical signals on a gene chip wherein an array of probes fabricated on said gene chip is hybridized with sample extractions labeled with fluorescence, which comprises:
- a titanium sapphire laser system to excite a light beam;
- a beam scanner receiving said light beam transmitted from said titanium sapphire laser system;
- a beam mirror means for amplifying and paralleling said light beam from said beam scanner;
- a dichroic mirror reflecting said light beam from said beam minor means;
- an objective focusing said light beam to a light spot for exciting said optical signals;
- said dichroic mirror separating the fluorescent light from said optical signals;
- a plurality of filters for filtering respective wavelengths of fluorescence of the fluorescent light;
- and
- a plurality of detection channels for detecting respective fluorescence simultaneously;
- a plurality of detection channels for detecting respective fluorescence simultaneously.
Dependencies are inferred from the claim text.