Patient classification
AssigneeBioseek, Inc.
InventorsEllen L. Berg, Eugene C. Butcher, Jennifer Melrose
Life of this patent
Estimating the term…
Abstract
Clinical patient tissue samples are classified according to the physiological status of cells present in the sample. In some embodiments of the invention, such cells are classified according to their ability to respond to therapeutic agents and treatments. In other embodiments, the cells or tissue samples are classified according to their status with respect to the activity of pathways of interest. The information thus derived is useful in prognosis and diagnosis, and can further be used develop surrogate markers for disease states, and to investigate the effect of genetic polymorphisms in the responsiveness and state of cells involved in disease.
Claims10
A method of preparing a biomap for the classification of a patient sample according to pathways associated with a disease state, the method comprising:
- contacting said patient sample in a test cell culture with a plurality of factors in an amount and incubating for a time sufficient to induce a plurality of pathways active in said cell culture;
- measuring at least four parameters selected from the group consisting of ICAM, VCAM, E-selectin, IL-8, Mig, HLA-DR, MCP-1, CD69, CD14, CD142, CD40, Eotaxin3, IL-1alpha, M-CSF, and CD38 and comparing the measurement of said at least four parameters with the measurement from a control cell culture, and
- recording said measurements of said at least four parameters to produce a biomap, wherein said biomap is indicative of the pathways that are active in said cell culture.
Dependencies are inferred from the claim text.