# US 6,762,292 B2 · Optimally labeled oligonucleotides

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- Number: US 6,762,292 B2
- Document ID: US6762292B2
- Status: Granted
- Kind: B2
- Application number: 09/911,039
- Filed: 2001-07-23
- Published: 2002-01-31
- Granted: 2004-07-13
- Earliest claimed: 1996-06-04
- Assignees: Poly Probe, Inc.
- Inventors: Thor W. Nilsen
- Web page: https://patentgrep.com/patent/US6762292B2

## CPC

- C12Q 1/6806: Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence)
- C12Q 1/682: Signal amplification
- C12Q 1/6841: In situ hybridisation
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

A method for the preparation of optimally labeled oligonucleotides wherein label-conjugated nucleotide triphosphates are incorporated into a nucleic acid sequence in a defined repetitive manner which allows for the optimal specific detectability of the oligonucleotide. The oligonucleotides of the present invention are useful in the assay of a wide variety of nucleic acid sequences, specifically wherever labeled nucleic acid probes are desired.

## Claims

### Claim 1

An oligonucleotide comprising a nucleotide sequence complementary to a primer, and a nucleotide repeat region downstream form said complementary sequence, wherein said nucleotide repeat region comprises at least one detectably labeled nucleotide, each of said at least one detectably labeled nucleotide containing the same detectable label, and wherein said nucleotide repeat region also comprises a plurality of nucleotides that are not detectably labeled.

### Claim 2 (depends on 1)

The oligonucleotide of claim 1, wherein said detectable label comprises a fluorescent dye.

### Claim 3 (depends on 1)

The oligonucleotide of claim 1, wherein said detectable label comprises a radiolabel.

### Claim 4 (depends on 3)

The oligonucleotide of claim 3, wherein said radiolabel is 32P.

### Claim 5 (depends on 1)

The oligonucleotide of claim 1, which is single-stranded.

### Claim 6 (depends on 1)

The oligonucleotide of claim 1, which is double-stranded.

### Claim 7 (depends on 1)

The oligonucleotide of claim 1, which contains from about 20 to about 1000 total nucleotides.

### Claim 8

An optionally labeled oligonucleotide, comprising a detectably labeled nucleic acid sequence and a nucleotide repeat region, wherein the nucleotide repeat region comprises at least one nucleotide that is conjugated to a detectable label, and a plurality of nucleotides that are not conjugated to a detectable label, wherein each of said at least one detectably labeled nucleotide contains the same detectable label.

### Claim 9 (depends on 8)

The oligonucleotide of claim 8, wherein said detectable label comprises a radiolabel.

### Claim 10 (depends on 9)

The oligonucleotide of claim 9, wherein said radiolabel is 32P.

### Claim 11 (depends on 8)

The oligonucleotide of claim 8, which is single-stranded.

### Claim 12 (depends on 8)

The oligonucleotide of claim 8, which is double-stranded.

### Claim 13 (depends on 8)

The oligonucleotide of claim 8, which contains from about 20 to about 1000 total nucleotides.

### Claim 14 (depends on 8)

The oligonucleotide of claim 8, wherein the detectable label comprises a fluorescent dye.

### Claim 15 (depends on 8)

The oligonucleotide of claim 8, wherein the detectable label comprises biotin.

### Claim 16 (depends on 8)

The oligonucleotide of claim 8, wherein the detectable label comprises digoxigenin.

### Claim 17 (depends on 8)

The oligonucleotide of claim 8, wherein the detectable label comprises an antibody.

### Claim 18 (depends on 8)

The oligonucleotide of claim 8, wherein the detectable label comprises an enzyme.

### Claim 19 (depends on 8)

The oligonucleotide of claim 8, wherein the detectable label comprises a receptor.

### Claim 20 (depends on 8)

The oligonucleotide of claim 8, wherein the detectable label comprises streptavidin.

### Claim 21 (depends on 8)

The oligonucleotide of claim 8, wherein the detectable label comprises avidin.