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US 6,762,048 B2Granted
System and apparatus for nucleic acid sequencing of single molecules by polymerase synthesis
AssigneeLI-COR, Inc.
InventorJohn G. K. Williams
Life of this patent
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Abstract
This invention relates to improved methods for sequencing and genotyping nucleic acid in a single molecule configuration. The method involves single molecule detection of fluorescent labeled PPi moieties released from NTPs as a polymerase extension product is created.
Claims21
An apparatus suitable for sequencing a target nucleic acid molecule comprising:
- a microstructure defining a flow chamber containing
- (a) a support, and
- (b) only one single individual immobilized molecule in single molecule configuration, said immobilized molecule selected from the group consisting of a nucleic acid polymerizing enzyme and said target nucleic acid;
- which microstructure is configured to permit labeled nucleotide analogs that are not positioned on the support to move through the flow chamber.
1 more element
An apparatus suitable for sequencing a target nucleic acid molecule comprising:
- a microstructure defining a flow chamber, said flow chamber divided into separate channels, each of which contains
- (a) a support, and
- (b) only one single individual immobilized molecule in single molecule configuration, said immobilized molecule selected from the group consisting of a nucleic acid polymerizing enzyme and said target nucleic acid;
- which microstructure is configured to permit labeled nucleotide analogs that are not positioned on the support to move through the flow chamber.
1 more element
An apparatus suitable for sequencing a target nucleic acid molecule comprising:
- a microstructure defining a flow chamber containing
- (a) a support having a fixed charge,
- (b) only one single individual immobilized molecule in single molecule configuration, said immobilized molecule selected from the group consisting of a nucleic acid polymerizing enzyme and said target nucleic acid, and
- (c) two electrodes positioned so as to provide a voltage gradient across said microstructure;
- which microstructure is configured to permit labeled nucleotide analogs that are not positioned on the support to move through the flow chamber.
2 more elements
Dependencies are inferred from the claim text.