Alginate layer system for chondrogenic differentiation of human mesenchymal stem cells
AssigneeOsiris Therapeutics, Inc.
InventorsKarl Kavalkovich, Raymond Boynton, Mary Murphy, Frank Barry
Life of this patent
Estimating the term…
Abstract
Disclosed are a composition of chemically defined components which support in vitro and in vivo chondrogenesis of mesenchymal stem cells, a method for in vitro and in vivo chondrogenic induction of such stem cells, and a method of forming human chondrocytes in vitro and in vivo from such stem cells.
Claims16
A composition for producing cartilage, comprising human mesenchymal stem cells in an alginate gel layer which supports the differentiation and maturation of human mesenchymal stem cells into chondrocytes and hyaluronic acid, and wherein the mesenchymal stem cells in the gel layer have been contacted with a chondroinductive agent.
A method for regenerating or repairing cartilage in an individual in need thereof comprising administering to said individual human a composition comprising mesenchymal stem cells in an alginate gel layer which supports the differentiation and maturation of human mesenchymal stem cells into a chondrogenic lineage to an extent sufficient to accelerate cartilage formation therefrom and hyaluronic acid, and wherein the mesenchymal stem cells in the gel layer have been contacted with a chondroinductive agent.
A method of forming cartilage in vitro, comprising:
- admixing human mesenchymal stem cells with a solution comprising an alginate and hyaluronic acid;
- polymerizing said alginate to form a composition comprising said human mesenchymal stem cells in an alginate gel layer;
- and
- contacting said human mesenchymal stem cells in the alginate gel layer with a chondroinductive agent.
Dependencies are inferred from the claim text.