Adeno-associated virus serotype 1 nucleic acid sequences, vectors and host cells containing same
AssigneeThe Trustees of the University of Pennsylvania
InventorsJames M. Wilson, Weidong Xiao
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Abstract
An improved tet-repressible system is described in which the transgene is expressed under the control of a promoter which is activated upon binding of a fusion protein composed of a reverse tet repressor/activation domain to tet operator sequences located immediately upstream of the transgene promoter. The tet operator sequences are substantially free of interferon inducible response elements (ISRE). The reverse tet repressor is fused to an activation domain which lacks signals for protein clearance, thus extending expression of the fusion protein. Suitably, the tetO sequences are immediately upstream of a quiet promoter.
Claims9
A recombinant virus having an AAV-1 capsid comprising an AAV-1 protein selected from among AAV-1 vp1 having the amino acid sequence of SEQ ID NO:13;
- AAV-1 vp2 having the amino acid sequence of SEQ ID NO:15and AAV-1 vp3 having the amino acid sequence of SEQ ID NO:17 and a heterologous molecule which comprises an AAV 5′ inverted terminal repeat sequence (ITR), a transgene, and an AAV 3′ ITR.
Dependencies are inferred from the claim text.