Method to prevent graft rejection using TGF-beta to induce T suppressor cells

A method for decreasing graft rejection of a solid organ by a recipient comprising:

• a) isolating peripheral blood mononuclear cells (PBMC) from a recipient and an organ donor;
• b) irradiating T cell-depleted mononuclear cells from said organ donor PBMC;
• c) combining ex vivo said recipient PBMC with said population of donor irradiated T cell-depleted mononuclear cells with a regulatory composition comprising TGF-β to induce a population of recipient suppressor T cells;
• d) expanding said population of recipient suppressor T cells;
• and
• e) administering said recipient suppressor T cells to said recipient.