Methods and compositions for organ decellularization
AssigneeChildren's Medical Center Corporation
InventorAnthony Atala
Life of this patent
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Abstract
The invention is directed to methods for producing a decellularized organ or part of an organ. A decellularized organ is produced using an isolated organ mechanically agitated to remove cellular membranes surrounding the isolated organ without destroying the interstitial structure of the organ. After the cellular membrane is removed, the isolated organ is exposed to a solubilizing fluid that extracts cellular material without dissolving the interstitial structure of the organ. A washing fluid is used to remove the solubilized components, leaving behind a decellularized organ.
Claims26
A method for producing a decellularized organ scaffold comprising:
- mechanically agitating an isolated untreated organ in membrane stripping fluid to disrupt cell membranes while maintaining the interstitial structure of the organ;
- treating the isolated organ in a solubilizing fluid at a concentration effective to extract cellular material from the organ while maintaining the interstitial structure of the organ;
- and
- washing the isolated organ in a washing fluid to remove cellular debris while maintaining the interstitial structure of the organ until the isolated organ is substantially free of cellular material, to thereby produce a decellularized organ scaffold.
A method for producing a decellularized blood vessel scaffold comprising:
- mechanically agitating an isolated untreated blood vessel in membrane stripping fluid to disrupt the outer part of the blood vessel while maintaining the interstitial structure of the blood vessel;
- treating the isolated blood vessel in a solubilizing fluid at a concentration effective to extract cellular material from the blood vessel while maintaining the interstitial structure of the blood vessel;
- and
- washing the isolated blood vessel in a washing fluid to remove cellular debris while maintaining the interstitial structure of the blood vessel until the isolated blood vessel is substantially free of cellular material, to thereby produce a decellularized blood vessel scaffold.
Dependencies are inferred from the claim text.