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US 6,747,737 B2Granted
Method for optical detection of an illuminated specimen in a plurality of detection channels
AssigneeCarl Zeiss Jena GmbH
InventorsRalf Wolleschensky, Gunter Moehler
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Abstract
In light detection systems for illuminating specimens having a plurality of detection channels, particularly channels employing laser scanning microscopes, an improved method comprises assigning an upper and/or lower limiting value which is adjustable for at least one channel and changing the channel to be detected with respect to its mode of operation when the limiting value is reached.
Claims13
A method for the optical detection of an illuminated specimen in a plurality of detection channels, comprising:
- sensing light on a detector with multiple detection channels with an illumination light scan pass so that repeated scanning is dispensed with;
- assigning an upper or lower limiting value or both values which is adjustable for each individual channel;
- and
- selecting the detection channel to be detected with respect to its mode of operation when said limiting value is reached during the illumination light scan pass.
1 more element
A method for controlling light to a laser scanning microscope comprising:
- performing an illumination scan pass;
- sending light from a specimen to a plurality of wavelength dependent detection channels and;
- determining which of the detection channels have light intensity above a set threshold present wherein only the scan pass is required so that the specimen is not overloaded with light and repeated scanning is dispensed with.
Dependencies are inferred from the claim text.