Method for the individual adaptation of excitation intensities in a multiband fluorescence microscope and multiband fluorescence microscope for carrying out said method

A multiband fluorescence microscope comprising an illumination beam path having a light source, a collector, a multiplicity of lens members, an aperture diaphragm, a radiant field diaphragm, an excitation filter for simultaneous production of several excitation bands of different light wavelengths and a filter element to affect the excitation bands, a beam splitter and an objective that directs the illumination beam path onto a fluorescence object on a specimen stage projecting it through the beam splitter, an output filter and a tube lens into an intermediate image plane, wherein

• a) a filter draw assembly made of individually movable, tightly spaced filter draws is inserted perpendicular in the illumination beam path tightly next to the aperture diaphragm,
• b) a selective filter is provided on each filter draw for each excitation band that has surface regions with high and low transmission factors,
• c) the surface region having the lowest transmission factor has a minimum diameter equal to the beam diameter (x) to completely cancel the excitation band,
• d) a blank aperture having the beam diameter (x) is arranged next to each filter,
• e) the transmission factor of each filter diminishes as one moves further away from the blank aperture, and
• f) separate positioning means, with which any surface region of the filter draw can be inserted in the illumination beam path, are assigned to each filter draw.