Phosphorylated fusion proteins
AssigneePestka Biomedical Laboratories, Inc.
InventorSidney Pestka
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Abstract
Modified proteins, modified interferons α's and β's, phosphorylated modified proteins and DNA sequences encoding the above, applications and uses thereof. Modified phosphorylated Hu-IFN-α-like proteins are provided which carry an identifiable label such as a radio-label. Corresponding phosphorylatable Hu-IFN-α-like proteins which contain a putative phosphorylation site. DNA sequences which encode a Hu-IFN-α-like protein and contain a sequence encoding a putative phosphorylatable site. Appropriate expression vectors are used to transform compatible host cells of various microorganisms, such as E. coli. Numerous uses for the phosphorylated proteins are disclosed.
Claims12
A phosphorylatable protein comprising an amino acid sequence of a protein not normally phosphorylated in vivo and having a desired bioactivity, which protein has been provided with a heterologeous phosphorylation recognition sequence for a kinasce wherein the phosphorylatable protein when phosphorylated on the phosphorylation recognition sequence retains the desired bioactivity.
Dependencies are inferred from the claim text.