Chemical modification of DNA using peptide nucleic acid conjugates
AssigneeIsis Pharmaceuticals, Inc.
InventorsPhilip L. Felgner, Olivier Zelphati, C. Frank Bennett
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Abstract
Complexes comprising a nucleic acid molecule and a conjugated peptide nucleic acid (PNA). The PNA may be labeled or conjugated to a protein, peptide, carbohydrate moiety or receptor ligand. These complexes are used to transfect cells to monitoring plasmid biodistribution, promote nuclear localization, induce transcriptional activation, lyse the endosomal compartment and facilitate transfection. These complexes increase the efficiency of expression of a particular gene.
Claims6
An in vitro method for delivery of an exogenous transfected nucleic acid molecule into a nuclear compartment of a cell comprising the steps of:
- hybridizing said exogenous nucleic acid molecule to a peptide nucleic acid (PNA) conjugated to a nuclear localization signal peptide, and transfecting said cell with said PNA hybridized nucleic acid molecule.
Dependencies are inferred from the claim text.