Method for cultivation of filamentous fungi
AssigneeFood Industry Research and Development Institute
InventorsWen-Teng Wu, Pei-Ming Wang, Ting-Kuo Huang, Gwo-Fang Yuan
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Abstract
The invention discloses a method for cultivation of filamentous fungi using a suspended nutritionally solid substrate, the method including the steps of: (a) preparing a medium comprising a nutritionally solid substrate; and (b) inoculating an inoculum into the medium comprising the nutritionally solid substrate in a bioreactor to carry out fermentation. The present invention combines the properties of solid-state culture and submerged culture. In addition, the present invention may employ an air-lift bioreactor with a net draft tube and the fed-batch process to elevate the yields of filamentous fungi and metabolites thereof.
Claims23
A method for cultivation of filamentous fungi comprising the steps of:
- (a) preparing a medium for submerged culture comprising rice particles that receive said filamentous fungi, wherein the rice particles are husked, cooked, and sterilized before being added to said medium;
- and
- (b) inoculating said medium with said filamentous fungi in a bioreactor to carry out fermentation wherein the mycelia of said filamentous fungi are attached to said rice particles.
A method for cultivation of the Monascus species comprising the steps of:
- (a) preparing a medium for submerged culture comprising rice particles that receives said Monascus species, wherein the rice particles are husked, cooked and sterilized before being added to said medium;
- and
- (h) inoculating said medium with said Monascus species in a bioreactor to carry out fermentation wherein the mycelia of said Monascus species are attached to said rice particles.
A method for producing metabolites from cultivation of the Monascus species comprising the steps of:
- (a) preparing a medium for submerged culture comprising rice particles that receive said Monascus species, wherein the rice particles are husked, cooked, and sterilized before being added to said medium;
- and
- (b) inoculating said medium with said Monascus species in a bioreactor to carry out fermentation wherein the mycelia of said Monascus species are attached to said rice particles.
Dependencies are inferred from the claim text.