FTA- coated media for use as a molecular diagnostic tool

A method of purifying nucleic acid comprising the steps of:

• a. providing a dry substrate comprising:
• i. a solid matrix, wherein the solid matrix comprises nitrocellulose or nylon;
• and
• ii. a coating, wherein the coating comprises an anionic surfactant or detergent which facilitates cellular lysis;
• b. applying to the substrate a sample comprising nucleic acid;
• c. capturing the nucleic acid with the substrate;
• d. optionally, fixing the nucleic acid to the substrate;
• e. treating the nucleic acid on the substrate with an external substance which generates a signal in an assay, wherein the assay comprises an enzyme-linked immunosorbant assay (ELISA);
• and
• f. generating a signal to indicate the presence of the nucleic acid.

A method of purifying nucleic acid comprising the steps of:

• a. providing a dry substrate comprising:
• i. a solid matrix, wherein the solid matrix comprises nitrocellulose or nylon;
• and
• ii. a coating, wherein the coating comprises an anionic surfactant or detergent which facilitates cellular lysis;
• b. applying to the substrate a sample comprising nucleic acid;
• c. capturing the nucleic acid with the substrate;
• d. optionally, fixing the nucleic acid to the substrate, wherein the fixing of the nucleic acid to the substrate comprises heating the substrate with the captured nucleic acid;
• e. treating the nucleic acid on the substrate with an external substance which generates a signal in an assay;
• and
• f. generating a signal to indicate the presence of the nucleic acid.

A method of purifying and analyzing DNA in a blood sample, wherein the method comprises the steps of:

• a. providing a dry substrate comprising:
• i. a solid matrix, wherein the solid matrix comprises nitrocellulose or nylon;
• and
• ii. a coating, wherein the coating comprises an anionic surfactant or detergent which facilitates cellular lysis;
• b. applying to the substrate a blood sample comprising DNA;
• c. capturing the DNA with the substrate;
• d. optionally, fixing the DNA to the substrate;
• e. treating the DNA which is maintained on the substrate with an external substance which generates a signal in an assay;
• f. generating a signal to indicate the presence of DNA captured and maintained on the substrate;
• g. analyzing the amount of DNA captured and maintained on the substrate by quantifying the generated signal;
• and
• h. analyzing the number of leukocytes in the blood sample by the amount of captured DNA analyzed by quantifying the generated signal.

A method of purifying and analyzing DNA in a blood sample, wherein the method comprises the steps of:

• a. providing a dry substrate comprising:
• i. a solid matrix, wherein the solid matrix comprises nitrocellulose or nylon;
• and
• ii. a coating, wherein the coating comprises an anionic surfactant or detergent which facilitates cellular lysis;
• b. applying to the substrate a blood sample comprising DNA;
• c. capturing the DNA with the substrate;
• d. optionally, fixing the DNA to the substrate;
• e. treating the DNA on the substrate with an external substance which generates a signal in an assay, wherein the external substance comprises polyethyleneimine;
• f. generating a signal to indicate the presence of DNA;
• and
• g. analyzing the amount of DNA captured the substrate by quantifying the generated signal.

A method of purifying and analyzing DNA in a blood sample, wherein the method comprises the steps of:

• a. providing a dry substrate comprising:
• i. a solid matrix, wherein the solid matrix comprises nitrocellulose or nylon;
• and
• ii. a coating, wherein the coating comprises an anionic surfactant or detergent which facilitates cellular lysis;
• b. applying to the substrate a blood sample comprising DNA;
• c. capturing the DNA with the substrate;
• d. optionally, fixing the DNA to the substrate;
• e. treating the DNA on the substrate with an external substance which generates a signal in an assay, wherein the assay comprises an enzyme-linked immunosorbant assay (ELISA);
• f. generating a signal to indicate the presence of DNA;
• and
• g. analyzing the amount of DNA captured by quantifying the generated signal.

A method of purifying and analyzing DNA in a blood sample, wherein the method comprises the steps of:

• a. providing a dry substrate comprising:
• i. a solid matrix, wherein the solid matrix comprises nitrocellulose or nylon;
• and
• ii. a coating, wherein the coating comprises an anionic surfactant or detergent which facilitates cellular lysis;
• b. applying to the substrate a blood sample comprising DNA;
• c. capturing the DNA with the substrate;
• d. optionally, fixing the DNA to the substrate, wherein the fixing of the DNA to the substrate comprises heating the substrate with the captured DNA;
• e. treating the DNA on the substrate with an external substance which generates a signal in an assay;
• f. generating a signal to indicate the presence of DNA;
• and
• g. analyzing the amount of DNA captured by quantifying the generated signal.

A method of purifying nucleic acid comprising the steps of:

• a. providing a dry substrate comprising:
• i. a solid matrix, wherein the solid matrix comprises nitrocellulose or nylon;
• and
• ii. a coating, wherein the coating comprises an anionic surfactant or detergent which facilitates cellular lysis;
• b. applying to the substrate a sample comprising nucleic acid;
• c. capturing the nucleic acid with the substrate;
• d. optionally, fixing the nucleic acid to the substrate;
• e. treating the nucleic acid which is maintained on the substrate with an external substance which generates a signal in an assay, wherein the external substance comprises polyethyleneimine;
• and
• f. generating a signal to indicate the presence of the nucleic acid which is maintained on the substrate.

A method of purifying nucleic acid comprising the steps of:

• a. providing a dry substrate comprising:
• i. a solid matrix, wherein the solid matrix comprises nitrocellulose or nylon;
• and
• ii. a coating, wherein the coating comprises an anionic surfactant or detergent which facilitates cellular lysis;
• b. applying to the substrate a sample comprising nucleic acid;
• c. capturing the nucleic acid with the substrate;
• d. optionally, fixing the nucleic acid to the substrate;
• e. treating the nucleic acid which is maintained on the substrate with an external substance which generates a signal in an assay, wherein the assay comprises an enzyme-linked immunosorbant assay (ELISA);
• and
• f. generating a signal to indicate the presence of the nucleic acid which is maintained on the substrate.

A method of purifying nucleic acid comprising the steps of:

• a. providing a dry substrate comprising:
• i. a solid matrix, wherein the solid matrix comprises nitrocellulose or nylon;
• and
• ii. a coating, wherein the coating comprises an anionic surfactant or detergent which facilitates cellular lysis;
• b. applying to the substrate a sample comprising nucleic acid;
• c. capturing the nucleic acid with the substrate;
• d. fixing the nucleic acid to the substrate wherein fixing comprises heating the substrate with the captured nucleic acid;
• e. treating the nucleic acid which is maintained on the substrate with an external substance which generates a signal in an assay;
• and
• f. generating a signal to indicate the presence of the nucleic acid which is maintained on the substrate.

A method of purifying and analyzing DNA in a blood sample, wherein the method comprises the steps of:

• a. providing a dry substrate comprising:
• i. a solid matrix, wherein the solid matrix comprises nitrocellulose or nylon;
• and
• ii. a coating, wherein the coating comprises anionic surfactant or detergent which facilitates cellular lysis;
• b. applying to the substrate a blood sample comprising DNA;
• c. capturing the DNA with the substrate;
• d. optionally, fixing the DNA to the substrate;
• e. treating the DNA which is maintained on the substrate with an external substance which generates a signal in an assay wherein the external substance comprises polyethyleneimine;
• f. generating a signal to indicate the presence of DNA captured and maintained on the substrate;
• and
• g. analyzing the amount of DNA captured and maintained on the substrate by quantifying the generated signal.

A method of purifying and analyzing DNA in a blood sample, wherein the method comprises the steps of:

• a. providing a dry substrate comprising:
• i. a solid matrix, wherein the solid matrix comprises nitrocellulose or nylon;
• and
• ii. a coating, wherein the coating comprises an anionic surfactant or detergent which facilitates cellular lysis;
• b. applying to the substrate a blood sample comprising DNA;
• c. capturing the DNA with the substrate;
• d. optionally, fixing the DNA to the substrate;
• e. treating the DNA which is maintained on the substrate with an external substance which generates a signal in an assay wherein the assay comprises an enzyme-linked immunosorbant assay (ELISA);
• f. generating a signal to indicate the presence of DNA captured and maintained on the substrate;
• and
• g. analyzing the amount of DNA captured and maintained on the substrate by quantifying the generated signal.

A method of purifying and analyzing DNA in a blood sample, wherein the method comprises the steps of:

• a. providing a dry substrate comprising:
• i. a solid matrix, wherein the solid matrix comprises nitrocellulose or nylon;
• and
• ii. a coating, wherein the coating comprises an anionic surfactant or detergent which facilitates cellular lysis;
• b. applying to the substrate a blood sample comprising DNA;
• c. capturing the DNA with the substrate;
• d. optionally, fixing the DNA to the substrate wherein fixing comprises heating the substrate with the captured nucleic acid;
• e. treating the DNA which is maintained on the substrate with an external substance which generates a signal in an assay;
• f. generating a signal to indicate the presence of DNA captured and maintained on the substrate;
• and
• g. analyzing the amount of DNA captured and maintained on the substrate by quantifying the generated signal.