Micro array and analyzing method using the same

A method for analyzing a biomolecule, comprising the steps of:

• (i) preparing a micro array, wherein said micro array comprises a stimulable phosphor layer provided on a substrate, wherein affixed within said phosphor layer is an array of a series of selected biomolecules, wherein the location of each selected biomolecule in said series, within said phosphor layer, is known,
• (ii) contacting the micro array of step (i) with a labeled biomolecule, to cause the labeled biomolecule to be bound to one or more members of the series of selected biomolecules, wherein said labeled biomolecule is labeled with an energy generating substance,
• (iii) exposing the resulting micro array of step (ii) to visible light to thereby induce the release of energy from phosphor molecules in the stimulable phosphor layer,
• (iv) placing the micro array of step (iii) in a dark place to thereby cause the stimulable phosphor layer to store energy released from the energy generating substance,
• (v) exposing the resulting micro array of step (iv) to stimulating rays which cause the stimulable phosphor layer to emit light in proportion to the amount of energy stored therein,
• (vi) photoelectrically detecting the resulting emitted light from step (v) as a signal, so as to detect the one or more members of the series of selected biomolecules which are bound to the labeled molecule, and
• (vii) determining the identity of the one or more members of the series of selected biomolecules bound to the labeled biomolecule by comparing the location of the detected signal in the micro array to the location of said one or more members of the series of selected biomolecules based on previously stored positional information.

A method for analyzing a biomolecule, comprising the steps of:

• (i) preparing a micro array, wherein said micro array comprises a stimulable phosphor layer provided on a substrate and a protective layer provided on said phosphor layer, wherein affixed within said protective layer is an array of a series of selected biomolecules, wherein the location of each selected biomolecule in said series, within said protective layer, is known,
• (ii) contacting the micro array of step (i) with a labeled biomolecule, to cause the labeled biomolecule to be bound to one or more members of the series of selected biomolecules, wherein said labeled biomolecule is labeled with an energy generating substance,
• (iii) exposing the resulting micro array of step (ii) to visible light to thereby induce the release of energy from phosphor molecules in the stimulable phosphor layer,
• (iv) placing the micro array of step (iii) in a dark place to thereby cause the stimulable phosphor layer to store energy released from the energy generating substance,
• (v) exposing the resulting micro array of step (iv) to stimulating rays which cause the stimulable phosphor layer to emit light in proportion to the amount of energy stored therein,
• (vi) photoelectrically detecting the resulting emitted light from step (v) as a signal, so as to detect the one or more members of the series of selected biomolecules which are bound to the labeled molecule, and
• (vii) determining the identity of the one or more members of the series of selected biomolecules bound to the labeled biomolecule by comparing the location of the detected signal in the micro array to the location of said one or more members of the series of selected biomolecules based on previously stored positional information.

A method for analyzing a sample, comprising the steps of:

• (i) preparing a micro array, wherein said micro array comprises a stimulable phosphor layer provided on a substrate, wherein affixed within said phosphor layer is an array of a series of selected detecting bodies, wherein the location of each selected detecting body in said series, within said phosphor layer, is known,
• (ii) contacting the micro array of step (i) with a sample, wherein said sample comprises a plurality of constituents which are labeled with an energy generating substance, to cause a constituent in said sample to be bound to one or more members of the series of selected detecting bodies,
• (iii) exposing the resulting micro array from step (ii) to visible light to thereby induce the release of energy from phosphor molecules in the stimulable phosphor layer,
• (iv) placing the micro array of step (iii) in a dark place to thereby cause the stimulable phosphor layer to store energy release from the energy generating substance,
• (v) exposing the resulting micro array of step (iv) to stimulating rays which cause the stimulable phosphor layer to emit light in proportion to the amount of energy stored therein,
• (vi) photoelectrically detecting the resulting emitted light from step (v) as a signal, so as to detect a labeled constituent of the sample which is bound to a detecting body, and
• (vii) determining the identity of a labeled constituent of the sample by comparing the location of the detected signal in the micro array to the location of said one or more members of the selected detecting bodies based on previously stored positional information.

A method for analyzing a sample, comprising the steps of:

• (i) preparing a micro array, wherein said micro array comprises a stimulable phosphor layer provided on a substrate and a protective layer provided on said phosphor layer, wherein affixed within said protective layer is an array of a series of selected detecting bodies, wherein the location of each selected detecting body in said series, within said protective layer, is known,
• (ii) contacting the micro array of step (i) with a sample, wherein said sample comprises a plurality of constituents which are labeled with an energy generating substance, to cause a constituent in said sample to be bound to one or more members of the series of selected detecting bodies,
• (iii) exposing the resulting micro array from step (ii) to visible light to thereby induce the release of energy from phosphor molecules in the stimulable phosphor layer,
• (iv) placing the micro array of step (iii) in a dark place to thereby cause the stimulable phosphor layer to store energy release from the energy generating substance,
• (v) exposing the resulting micro array of step (iv) to stimulating rays which cause the stimulable phosphor layer to emit light in proportion to the amount of energy stored therein,
• (vi) photoelectrically detecting the resulting emitted light from step (v) as a signal, so as to detect a labeled constituent of the sample which is bound to a detecting body, and
• (vii) determining the identity of a labeled constituent of the sample by comparing the location of the detected signal in the micro array to the location of said one or more members of the selected detecting bodies based on previously stored positional information.