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US 6,746,837 B2Granted
Multiple drug resistance gene atrD of aspergillus nidulans
AssigneeStichting voor de Technische Wetenschappen
InventorMaarten A. de Waard
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Abstract
The invention provides isolated nucleic acid compounds encoding a multiple drug resistance protein of Aspergillus nidulans. Vectors and transformed host cells comprising the multiple drug resistance-encoding DNA of Aspergillus nidulans atrD are also provided. The invention further provides assays which utilize these transformed host cells.
Claims3
A method for determining the fungal multiple drug resistance (MDR) inhibition activity of a compound which comprises:
- a) placing a culture of fungal cells, transformed with a vector which expresses a nucleic acid encoding an atrD protein consisting essentially of the amino acid sequence of SEQ ID NO:2, in the presence of:
- (i) an antifungal agent to which said fungal cell is resistant, but to which said fungal cell is sensitive in its untransformed state;
- (ii) a compound suspected of possessing Aspergillus nidulans MDR inhibition activity;
- and
- b) determining the fungal MDR inhibition activity of said compound by measuring the ability of the antifungal agent to inhibit the growth of said fungal cell.
2 more elements
Dependencies are inferred from the claim text.