Isolated bifidobacteria that produce siderophores which inhibit growth of lactococcus lactis
AssigneeRegents of the University of Minnesota
InventorDaniel J. O'Sullivan
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Abstract
Isolated Bifidobacterium strains are obtained that secrete a siderophore that inhibits the growth of microbes in the gastrointestinal tract of an animal. Microbes inhibited include Lactococcus lactis, Clostridium difficile, and Clostridium perfringens. The Bifidobacterium or siderophore isolated from the Bifidobacterium can be administered to an animal. The Bifidobacterium is grown under iron limiting conditions when obtaining the siderophore. Microbes are inhibited by the siderophore binding iron.
Claims18
A composition consisting essentially of an isolated Bifidobacterium that secretes a siderophore that inhibits growth of a Lactococcus lactis.
A composition comprising an isolated Bifidobacterium that secretes a siderophore that inhibits growth of a Lactococcus lactis, wherein the composition comprises substantially no free iron, comprises an iron chelator, or a combination thereof.
A composition comprising an isolated Bifidobacterium that secretes a siderophore which inhibits growth of a Lactococcus lactis, and a food wherein said composition is substantially free of iron, comprises an iron chelator, or a combination thereof.
A method for establishing a Bifidobacterium flora in the gastrointestinal tract of an animal comprising administering to an animal a composition comprising an isolated Bifidobacterium that secretes a siderophore that inhibits the growth of Lactococcus lactis, wherein the composition comprises substantially no free iron, comprises an iron chelator, or a combination thereof, and measuring the presence of the Bifidobacterium in the gastrointestinal tract of the animal after administration.
A method for preventing the replication of microbes selected from the group consisting of Lactococcus lactis, Clostridium difficile and Clostridium perfringens in a food, the method comprising adding to the food a composition comprising an isolated Bifidobacterium that secretes siderophore that inhibits growth of Lactococcus lactis, wherein the composition comprises substantially no free iron, comprises an iron chelator, or a combination thereof.
A method for obtaining a secreted siderophore that inhibits the growth of Lactococcus lactis from an isolated Bifidobacterium, the method comprising incubating the isolated Bifidobacterium under iron limited conditions, and isolating the siderophore.
A method for inhibiting the replication of a microbe selected from the group consisting of Lactococcus lactis, Clostridium difficile and Clostridium perfringens in the gastrointestinal tract of an animal, comprising administering to an animal a composition comprising an isolated Bifidobacterium that secretes a siderophore that inhibits growth of Lactococcus lactis, wherein the composition comprises substantially no free iron, comprises an iron chelator, or a combination thereof, and measuring the presence of said microbe that was present in the gastrointestinal tract of the animal prior to administration, where a decrease in the presence of the microbe in the animal after administration of the Bifidobacterium indicates inhibition of the replication of the microbe.
A method for inhibiting the replication of a microbe selected from the group consisting of Lactococcus lactis, Clostridium difficile and Clostridium perfringens, in a composition, the method comprising adding to the composition a secreted siderophore that inhibits the growth of Lactococcus lactis obtained from an isolated Bifidobacterium.
Dependencies are inferred from the claim text.