Reaction chamber roll pump
AssigneeAgilent Technologies, Inc.
InventorJohn F. McEntee
Life of this patent
Estimating the term…
Abstract
A method and system for circulating sample solution within a reaction chamber containing a microarray. The reaction chamber contains, on each side, a shallow vertical and a deep vertical well at the corners of the microarray. The vertical wells having a gap between the active surface of the microarray and the bottom of the reaction chamber are filled with sample solution. As the reaction chamber is rotated, sample solution from the deep vertical well displaces sample solution in the gap between the active surface of the microarray and the bottom of the reaction vessel, and sample solution from that gap is, in turn, displaced into the shallow vertical well, from which it flows along the inner surface of a cover strip above the microarray back to the deep vertical well.
Claims26
A roll pump within an enclosed region comprising:
- a first solution-collection region;
- a solution chamber, in communication with the first solution-collection region, in which solution is held within the chamber by capillary action;
- a second solution-collection region in communication with the solution chamber;
- and
- a solution-return surface on which solution moves from the second solution-collection region to the first solution-collection region.
A roll pump incorporated within an enclosed reaction chamber, the roll pump comprising:
- a first well near a first side of the reaction chamber, the first side of the reaction chamber substantially parallel with a rotation axis about which the reaction chamber is rotated;
- a gap between a lower surface of a reactive entity enclosed within the reaction chamber and an inner, bottom surface of the reaction chamber maintained by positioning features within the reaction chamber;
- and
- a gap along an inner surface of a top wall of the reaction chamber, the first well, the gap between the lower surface of the reactive entity and the inner, bottom surface of the reaction chamber, and the gap along the inner surface of the top wall of the reaction chamber forming a continuous, enclosed volume around the reactive entity through which solution circulates as the reaction chamber is rotated about the rotation axis.
A roll pump incorporated within an enclosed reaction chamber containing a microarray, the roll pump comprising:
- a deep well at a first side of the reaction chamber substantially parallel with a rotation axis about which the reaction chamber is rotated;
- a shallow well at a second side of the reaction chamber substantially parallel with the rotation axis;
- a gap between an active surface of the microarray and an inner, bottom surface of the reaction chamber maintained by positioning features within the reaction chamber;
- a gap along an inner surface of a top wall of the reaction chamber, the deep well, shallow well, the gap between the active surface of the microarray and the inner, bottom surface of the reaction chamber, and the gap along the inner surface of the top wall of the reaction chamber forming a continuous, enclosed volume around the microarray through which solution circulates as the reaction chamber is rotated about the rotation axis.
Dependencies are inferred from the claim text.