Use of an adsorbent gel for eliminating and purifying biomolecules
AssigneeCentre National De La Recherche Scientifique
InventorsMookambeswaran Vijayalakshmi, Olivier Pitiot, Cécile Legallais, Philippe Moriniere
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Abstract
The invention concerns the use of an adsorptive size exclusion chromatography gel, said gel essentially consisting of a polysaccharide matrix whereon is grafted a polymer coupled with an affinity ligand and having a cleavage threshold ranging between 2 kDa and 60 kDa for eliminating a purifying biomolecules.
Claims12
A device for removing biomolecules comprising an ultrafiltration module optionally upstream and in series with a dialysis module, wherein this device further comprises a column containing an adsorbent gel combining the properties of size exclusion and affinity chromatographies, said adsorbent gel consisting essentially of a polysaccharide matrix onto which is grafted a polymer coupled to an affinity ligand and having an adjustable cut-off of between 2 kDa and 60 kDa, said column being mounted branching from said ultrafiltration module.
A device for separating and purifying biomolecules comprising a column containing an adsorbent gel combining the properties of size exclusion and affinity chromatographies, said gel consisting essentially of a polysaccharide matrix onto which is grafted a polymer coupled to an affinity ligand and having an adjustable cut-off of between 2 kDa and 60 kDa, said column being mounted branching from a ultrafiltration module.
Dependencies are inferred from the claim text.