Method to produce succinic acid from raw hydrolysates
AssigneeThe University of Chicago
InventorsMark I. Donnelly, Cynthia Y. Sanville-Millard, Nhuan Phu Nghiem
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Abstract
A method for producing succinic acid from industrial-grade hydrolysates is provided, comprising supplying an organism that contains mutations for the genes ptsG, pflB, and ldhA, allowing said organism to accumulate biomass, and allowing said organism to metabolize the hydrolysate. Also provided is a bacteria mutant characterized in that it produces succinic acid from substrate contained in industrial-grade hydrolysate in a ratio of between 0.6:1 and 1.3:1 succinic acid to substrate.
Claims17
A method of producing succinic acid from plant hydrolysates comprising:
- a) supplying an organism, selected from the group consisting of AFP 184, AFP 400 and AFP 404;
- b) contacting said organism with hydrolysates said;
- c) allowing said organism to metabolize said hydrolysates;
- and
- d) isolating and recovering the succinic acid from the organism.
A method of producing succinic acid from plant hyrdolysates comprising:
- a) supplying an organism from a genus of Escherichia coli, said organism containing mutations for the genes ptsG, pflB, and IdhA, wherein the mutations cause the organism to contain an inoperative phosphotransferase system, an inoperative pyruvate formate lyase system and an inoperative lactate dehydrogenase system;
- b) contacting said organism with said hydrolysate:
- c) allowing said organism to metabolize said hydrolysate;
- and
- d) isolating and recovering the succinic acid from the claimed organism.
Dependencies are inferred from the claim text.