Linoleate isomerase
AssigneeArkion Life Sciences, LLC
InventorsReinhardt A. Rosson, Alan D. Grund, Ming-De Deng, Fernando Sanchez-Riera
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Abstract
The present invention provides an isolated linoleate isomerase and its nucleic acid and amino acid sequence. The present invention also provides a method for producing CLA from an oil using an immobilized bacterial cell or an isolated linoleate isomerase.
Claims47
An isolated linoleate isomerase from a bacterium selected from the group consisting of Lactobacillus, Clostridium, Propionibacterium, Butyrivibrio, and Eubacterium having characteristics comprising:
- a. a molecular weight of between about 50 kDa and about 70 kDa;
- b. an optimum pH of from about 6.8 to about 9.0;
- and,
- c. a Km of at least about 8.1 μM for linoleic acid at pH 7.5 and at a temperature of about 20° C.,
- wherein said isolated linoleate isomerase has been isolated from a cell as a soluble or solubilized enzyme preparation, and wherein said isolated linoleate isomerase has linoleate isomerase enzymatic activity.
An isolated linoleate isomerase comprising an amino acid sequence selected from the group consisting of:
- a. an amino acid sequence selected from the group consisting of SEQ ID NO:9, SEQ ID NO:11 and SEQ ID NO:18;
- and
- b. an amino acid sequence that is at least 90% identical to an amino acid sequence selected from the group consisting of SEQ ID NO:9, SEQ ID NO:11 and SEQ ID NO:18;
- wherein said isolated linoleate isomerase has linoleate isomerase enzymatic activity.
An isolated linoleate isomerase comprising an amino acid sequence selected from the group consisting of:
- a. an amino acid sequence selected from the group consisting of:
- SEQ ID NO:
- 9, SEQ ID NO:11, SEQ ID NO:18;
- and
- b. enzymatically active fragments of said amino acid sequence of (a);
- wherein said isolated linoleate isomerase has linoleate isomerase enzymatic activity.
Dependencies are inferred from the claim text.