Method of making microencapsulated DNA for vaccination and gene therapy
AssigneeMicrobiological Research Authority
InventorsDavid Hugh Jones, Graham Henry Farrar, James Christopher Stephen Clegg
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Abstract
A method of making a microparticle that contains DNA coding for a polypeptide is described in which a solvent extraction method is used and solvent extraction takes place at elevated temperature. Oral administration of the microparticle leads to its expression. DNA coding for an immunogen is for stimulating antibody formation in a recipient and DNA coding for a non-immunogenic polypeptide is for gene therapy applications. DNA is incorporated into the microparticle without destruction of its function.
Claims26
A composition comprising a polymer microparticle and an aqueous solution of DNA, wherein the DNA comprises a coding sequence, wherein the microparticle is 10 μm or less in diameter, and wherein the aqueous solution of DNA has an alcohol content of 1 to 40% and is encapsulated inside the microparticle.
A pharmaceutical composition comprising a plurality of polymer microparticles and a pharmaceutically acceptable carrier, wherein the microparticles contain an aqueous solution of DNA the aqueous solution of DNA has an alcohol content of 1 to 40%, and the DNA comprises a coding sequence encoding a polypeptide selected from the group consisting of:
- (a) the antigens FHA, PT, 68 kd-Pertacin, tetanus toxin, gp-48, NS1, Capsid, gp350, NS3, SA, I, NP E, M, gp340, F, H, HN, 35 kd protein, VP1, E1, E2, C, M, E and MSHA;
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- (b) immunogenic fragments, of the polypeptides of (a).
Dependencies are inferred from the claim text.