Loading patent…
AssigneeCollagen Matrix Technologies, Inc.
InventorsDale P. DeVore, Peter D. Ciarametaro
Estimating the term…
Methods are provided for processing and dispersing animal tissues that involve exposing processed (e.g., decellularized) tissue to an acylating agent, wherein the ratio of acylating agent to wet tissue weight is about 0.003:1 or less. Preferably, decellularized tissue is exposed to an amount of the acylating agent of about 0.1% to about 0.3% of wet tissue weight for a time ranging from about 30 seconds to about 10 minutes. A dispersed tissue matrix is produced that has a high degree of resistance to digestion by non-collagenase proteases, such as trypsin. In order to produce sufficiently high yields of these trypsin-resistant compositions, the processed tissue is preferably cryomilled to increase its surface area prior to acylation. The dispersed acylated decellularized tissue may be in the form of an injectable composition that can be introduced into tissue of a subject.
A method for processing dermal tissue for implantation into a subject, said method comprising the steps of:
A method for dispersing decellularized animal connective tissue, said method comprising the steps of:
A method for augmenting the condition of in situ tissue of a subject, said method comprising introducing an effective amount of a dispersed collagen matrix into said in situ tissue of said subject, said dispersed collagen matrix being prepared by treating a decellularized animal connective tissue matrix to cause a reduction in size and an increase in surface area and contacting said treated decellularized, animal connective tissue matrix with a solution comprising an acylating agent for a time ranging from about 30 seconds to about 10 minutes, wherein the amount of said acylating agent is about 0.1% to about 0.3% of wet tissue weight.
A composition comprising an injectable, dispersed collagen matrix prepared by treating a decellularized animal connective tissue matrix to cause a reduction in size and an increase in surface area and contacting said treated decellularized animal connective tissue with a solution comprising an acylating agent for a time ranging from about 30 seconds to about 10 minutes, wherein the amount of said acylating agent is about 0.1% to about 0.3% of wet tissue weight.
An injectable composition comprising decellularized, acylated, dispersed, dermal tissue matrix comprising a collagen matrix, said tissue being acylated with an acylating agent in an amount of about 0.1% to about 0.3% of wet tissue weight for a time ranging from about 30 seconds to about 10 minutes and having a trypsin resistance such that greater than about 40% of the collagen matrix remains undigested when exposed to 2% trypsin at 37° C. for 6-24 hours.
Dependencies are inferred from the claim text.