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US 6,740,517 B2Granted
Incubation method for obtaining solid culture of Zang Zhi, solid culture obtained therefrom, processed products and use thereof
OwnerMing-Huang Lan, Li-Yu Wuinventors
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Abstract
A solid culture of Zang Zhi (Antrodia camphorata) having triterpenoid compounds, water-soluble polysaccharides and chitin. The solid culture is prepared by a method including the steps of: incubating a stock strain of Zang Zhi in a bag for growth of mycelia, followed by an incubation in the air for fruiting the Zang Zhi body.
Claims4
A cultured fungus product of Antrodia camphorata prepared by a process comprising:
- (a) culturing spawn of a wild-type fungus of Antrodia camphorata in a bag containing a medium comprising 10-70% of a cellulolytic substance, 10-30% of a starch, 5-15% of millet, 1-10% of a saccharide, 0.5-2% of a phosphate and 0.1-1% of a sulfite salt at a relative humidity of 60-80%, the medium having a neutral pH, the culturing being carried out at a temperature of 5-32° C. in an atmosphere comprising 0.1-1% carbon dioxide;
- (b) removing the medium from the bag;
- (c) exposing the removed medium to air and maintaining the removed medium in the air for a period of days until a solid fruiting body is formed of conoid shape, said removed medium being maintained in the air at a temperature which differs between days and nights with the removed medium being maintained during the days at a temperature between 20° and 30° C. and during the nights at a temperature of 10-12° C., wherein the humidity of the air is between 90-95% and the air contains less than 1% of carbon dioxide;
- and
- (d) recovering the solid fruiting body of conoid shape.
2 more elements
Dependencies are inferred from the claim text.