Modified phytases
AssigneeRoche Vitamins Inc.
InventorsDirk Kostrewa, Luis Pasamontes, Andrea Tomschy, Adolphus van Loon, Kurt Vogel, Markus Wyss
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Abstract
A process for the production of a modified phytase with a desired property improved over the property of the corresponding unmodified phytase is disclosed, as well as modified phytases, polynucleotides encoding modified phytases, and animal feed including modified phytases.
Claims31
A polynucleotide comprising a DNA sequence coding for a modified Aspergillus fumigatus phytase with a specific activity improved over the specific activity of the corresponding unmodified Aspergillus fumigatus phytase wherein the amino acid sequence of the unmodified phytase has been changed at a position corresponding to position 27 of the phytase of Aspergillus niger (SEQ ID NO:1), as identified by PILEUP version 8 amino acid sequence alignment program, to an amino acid selected from the group consisting of Ala, Val, Leu, Ile, Thr, Gly, and Asn.
A polynucleotide comprising a DNA sequence coding for a modified Aspergillus fumigatus phytase with a specific activity improved over the specific activity of the corresponding unmodified Aspergillus fumigatus phytase wherein the amino acid sequence of the modified Aspergillus fumigatus phytase has a mutation selected from the group consisting of S66D, S140Y, D141G, A205E, Q274L, G277D, G277K, Y282H, N340S, and combinations thereof, wherein the respective amino acid position of each mutation corresponds to the amino acid position of an Aspergillus niger phytase (SEQ ID NO:1) as identified by PILEUP version 8 amino acid alignment program.
Dependencies are inferred from the claim text.