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US 6,733,977 B2Granted
Hybridization device and method
AssigneeAffymetrix, Inc.
InventorsDonald M. Besemer, Virginia W. Goss, James L. Winkler
Life of this patent
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Abstract
A body 300 having a cavity 310 for mounting a substrate 120 fabricated with probe sequences at known locations according to the methods disclosed in U.S. Pat. No. 5,143,854 and PCT WO 92/10092 or others, is provided. The cavity includes inlets 350 and 360 for introducing selected fluids into the cavity to contact the probes. Accordingly, a commercially feasible device for use in high throughput assay systems is provided.
Claims30
An apparatus for hybridization of probe arrays, comprising:
- a substrate comprising a first surface and a second surface, said first surface comprising said probe array of biological polymers disposed thereon, said probe array comprising greater than 100 different probes at known locations on said first surface;
- and
- a housing comprising a mounting surface and a fluid cavity;
- said fluid cavity comprising an inlet port constructed to permit fluid flow into said cavity through said inlet port, wherein said first surface of said substrate is sealably mounted with respect to said mounting surface thereby sealably covering said cavity and whereby said probe array is located inside said cavity.
1 more element
A method of hybridizing probe arrays comprising the acts of:
- providing a substrate comprising a first surface and a second surface, said first surface comprising said probe array of biological polymers disposed thereon, said probe array comprising greater than 100 different probes at known locations on said first surface;
- sealably mounting said substrate to a housing comprising a mounting surface and a fluid cavity;
- said fluid cavity comprising an inlet port constructed to permit fluid flow into said cavity through said inlet port, wherein said sealably mounting act provides said probe array located inside said cavity;
- and
- introducing said fluid inside said cavity to hybridize said probe array of biological polymers.
2 more elements
Dependencies are inferred from the claim text.