Vectors in avian transgenesis
AssigneeAviGenics, Inc.
InventorsRobert D. Ivarie, Alex J. Harvey, Julie A. Morris, Guodong Liu, Jeffrey C. Rapp
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Abstract
This invention provides vectors and methods for the stable introduction of exogenous nucleic acid sequences into the genome of a bird and for expressing said exogenous sequences to alter the phenotype of the bird or to produce desired proteins. In particular, transgenic chickens are produced which express exogenous sequences in their oviducts. Eggs which contain exogenous proteins are also produced.
Claims7
A method for producing an exogenous interferon α or erythropoietin protein in an egg of a chicken, which method comprises:
- a) providing an avian leukosis viral vector comprising a nucleic acid sequence encoding an exogenous interferon a or erythropoietin protein, and a constitutive promoter operably linked to said sequence, wherein said promoter drives expression of the encoding sequence in the chicken oviduct;
- b) introducing said vector into chicken stage X embryonic cells;
- c) incubating said embryonic cells under conditions conducive to hatching live chicks;
- d) nurturing growth of a mature chimeric chicken from said chicks;
- e) mating said chimeric chicken, either naturally or via artificial insemination, with a non-transgenic chicken;
- f) identifying a transgenic chicken by screening the progeny of step e) for germ line incorporation of the protein encoding sequence;
- and
- g) mating the transgenic progeny with non-transgenic chickens to produce eggs containing the exogenous protein.
A method for producing an exogenous interferon α or erythropoietin protein in an egg of a chicken, which method comprises:
- a) providing an avian leukosis viral vector comprising a nucleic acid sequence encoding an exogenous interferon α or erythropoietin protein, and a constitutive promoter operably linked to said sequence, wherein said promoter drives expression of the encoding sequence in the chicken oviduct;
- b) introducing said vector into chicken stage X embryonic cells;
- c) incubating said embryonic cells under conditions conducive to hatching live chicks;
- d) nurturing growth of a mature chimeric chicken from said chicks;
- e) mating said chimeric chicken, either naturally or via artificial insemination, with a non-transgenic chicken;
- f) identifying a transgenic chicken by screening the progeny of step e) for germ line incorporation of the protein encoding sequence;
- g) mating the transgenic progeny with non-transgenic chickens to produce said egg containing the exogenous protein;
- and
- h) extracting the exogenous protein from the egg.
Dependencies are inferred from the claim text.