Fructosyltransferases
AssigneeNederlandse Organisatie voor toegepast-natuurwetenschappelijk onderzoek TNO
InventorsSacha Adrianus Fokke Taco Van Hijum, Gerritdina Hendrika Van Geel-Schutten, Lubbert Dijkhuizen, Hakim Rahaoui
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Abstract
The present invention describes two novel proteins having fructosyltransferase activity. Both enzymes are derived from lactobacilli, which are food-grade micro-organisms with the Generally Recognized As Safe (GRAS) status. One of these proteins produces an inulin and fructo-oligosaccharides, while the other produces a levan and fructo-oligosaccharides. According to the invention lactobacilli capable of producing an inulin and/or a levan and/or fructo-oligosaccharides using one or both of the fructosyltransferases can be used as a probiotic or a symbiotic.
Claims8
A process of producing a fructo-oligosaccharide or fructo-polysaccharide, having β(2-1) linked D-fructosyl units comprising forming a mixture by combining sucrose with at least one reaction partner selected from the group consisting of:
- a) a protein having fructosyltransferase activity, which exhibits at least 85% amino acid identity, as determined by a BLAST algorithm, with an amino acid sequence of SEQ ID No. 1, and
- b) a recombinant host cell containing one or more copies of a nucleic acid construct encoding for said protein (a) and capable of expressing said protein;
- wherein said reaction partner interacts with sucrose to produce a fructo-oligosaccharide or fructo-polysaccharide.
A process of producing a fructo-oligosaccharide or fructo-polysaccharide, having β(2-1) linked D-fructosyl units comprising combining sucrose and a protein to form a mixture, said protein having fructosyltransferase activity, which exhibits at least 85% amino acid identity, as determined by a BLAST algorithm, with an amino acid sequence of SEQ ID No. 1, and
- interacting said sucrose with said protein to produce said fruco-oligosaccharide or fructo-polysaccharide.
A process for producing a fructo-oligosaccharide or fructo-polysaccharide, having β(2-6) linked D-fructosyl units comprising forming a mixture by combining sucrose with a reaction partner, wherein said reaction partner is a recombinant host cell containing one or more copies of a nucleic acid construct encoding for a protein having fructosyltransferase activity, which exhibits at least 85% amino acid identity, as determined by a BLAST algorithm, with an amino acid sequence of SEQ ID No. 11, and wherein said reaction partner interacts with sucrose to provide a fructo-oligosaccharide or fructo-polysaccharide.
Dependencies are inferred from the claim text.