Protein capable of catalyzing transamination stereoselectively, gene encoding said protein and use thereof
AssigneeSumitomo Chemical Company, Limited
InventorsYoshiki Takashima, Satoshi Mitsuda, Marco Wieser
Life of this patent
Estimating the term…
Abstract
DNA encoding a novel protein from Mycobacterium aurum SC-S423 capable of converting acetophenone to an optically active 1-phenylethlamine in the presence of a racemic mixture of sec-butylamine is provided, along with a vector and host cell comprising the same and a method of producing the same.
Claims13
An isolated DNA molecule encoding a protein comprising an amino acid sequence selected from the group consisting of:
- (a) an amino acid sequence represented by SEQ ID NO:1;
- (b) an amino acid sequence represented by SEQ ID NO:1, which contains a single amino acid substitution therein, wherein said protein converts acetophenone to an optically active 1-phenylehtylamine in the presence of a racemic mixture of sec-butylamine;
- (c) an amino acid sequence encoded by nucleotides 1 to 1017 of the nucleotide sequence represented by SEQ ID NO:2;
- (d) an amino acid sequence having 90% or greater amino acid identity with the amino acid sequence represented by SEQ ID NO:1 wherein said protein converts acetophenone to an optically active 1-phenylethylamine in the presence of a racemic mixture of sec-butylamine;
- (e) an amino acid sequence having 90% or greater amino acid sequence identity with the amino acid sequence represented by SEQ ID NO:1, wherein said protein converts acetophenone to an optically active 1-phenylethylamine in the presence of a racemic mixture of sec-butylamine and has a molecular weight of about 37 kDa as a monomer;
- and
- (f) an amino acid sequence having 90% or greater amino acid identity with the amino acid sequence represented by SEQ ID NO:1, wherein said protein converts acetophenone to an optically active 1-phenylehtylamine in the presence of a racemic mixture of sec-butylamine, and wherein said protein is obtainable from a Mycobacterium.
An isolated DNA molecule comprising a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence comprising nucleotides 1 to 1017 of the nucleotide sequence represented by SEQ ID NO:2;
- and,
- (b) a nucleotide sequence comprising nucleotides 1 to 1017 of the nucleotide sequence represented by SEQ ID NO:2, which contains a nucleotide substitution of adenine to guanine at nucleotide 4;
- (c) a nucleotide sequence of about 1020 bp which is amplified by PCR using as primers:
- (i) an oligonucleotide comprising nucleotides 1 to 28 of the nucleotide sequence represented by SEQ ID NO:2;
- or an oligonucleotide comprising the nucleotide sequence represented by SEQ ID NO:11, and
- (ii) an oligonucleotide comprising a nucleotide sequence complementary to the nucleotide sequence comprising nucleotides 999 to 1020 of the nucleotide sequence represented by SEQ ID NO:2, and as a template,
- (iii) chromosomal DNA obtained from a Mycobacterium and which encodes a protein which converts acetophenone to an optically active 1-phenylethylamine in the presence of a racemic mixture of sec-butylamine.
An isolated DNA molecule comprising nucleotides 1 to 1017 of the nucleotide sequence represented by SEQ ID NO:2.
An isolated DNA molecule comprising nucleotides 1 to 1017 of the nucleotide sequence represented by SEQ ID NO:2, which contains a nucleotide substitution of adenine to guanine at nucleotide 4.
Dependencies are inferred from the claim text.