Plants containing a cytosolic acetyl CoA-carboxylase nucleic acid
AssigneeCargill, Incorporated
InventorsLorin R. DeBonte, Basil S. Shorrosh
Life of this patent
Estimating the term…
Abstract
Plants are disclosed that contain a recombinant nucleic acid construct comprising a nucleic acid encoding a cytosolic acetyl coA-carboxylase (ACCase) operably linked to a promoter. Seeds produced from such plants exhibit statistically significantly increased oil content as compared to seeds produced by a corresponding plant lacking the nucleic acid encoding the ACCase. Methods of producing seeds exhibiting statistically significantly increased oil content are also disclosed.
Claims22
A plant containing a recombinant nucleic acid construct comprising a nucleic acid encoding a cytosolic ACCase operably linked to a promoter, wherein said construct lacks a nucleic acid encoding a transit peptide operably linked to said nucleic acid encoding said cytosolic ACCase, wherein said plant produces seeds that exhibit a statistically significant increase in oil content as compared to seeds produced by a corresponding plant lacking said nucleic acid construct, wherein said increase in oil content is from about 5% to about 25% on a dry weight basis.
A method of producing a plant, comprising:
- (a) providing a plant comprising a nucleic acid construct comprising a nucleic acid encoding a cytosolic ACCase operably linked to a promoter, wherein said construct lacks a nucleic acid encoding a transit peptide operably linked to said nucleic acid encoding said cytosolic ACCase;
- and
- (b) selecting, for at least one generation, progeny plants that produce seeds exhibiting a statistically significant increase in oil content as compared to seeds produced by a corresponding plant lacking said nucleic acid construct, wherein said increase in oil content is from about 5% to about 25% on a dry weight basis.
A method of producing a plant, comprising the step of:
- (a) introducing a construct into one or more plants, said construct comprising a nucleic acid encoding a cytosolic acetyl ACCase operably linked to a promoter, wherein said construct lacks a nucleic acid encoding a transit peptide operably linked to said nucleic acid encoding said cytosolic ACCase,
- wherein progeny of one or more of said transgenic plants, following at least one generation of selection, produce seeds that exhibit a statistically significant increase in oil content as compared to seeds produced by a corresponding plant lacking said nucleic acid encoding said ACCase, wherein said increase in oil content is from about 5% to about 25% on a dry weight basis.
A method of increasing the oil content in seeds, comprising the steps of:
- (a) creating one or more plants containing a nucleic acid construct, said nucleic acid construct comprising a nucleic acid encoding a cytosolic ACCase operably linked to a promoter, wherein said construct lacks a nucleic acid encoding a transit peptide operably linked to said nucleic acid encoding said cytosolic ACCase;
- and
- (b) selecting progeny of said one or more plants that exhibit a statistically significant increase in oil content in seeds as compared to seeds produced by a corresponding plant lacking said nucleic acid encoding said ACCase, wherein said increase in oil content is from about 5% to about 25% on a dry weight basis.
Dependencies are inferred from the claim text.