# US 6,723,708 B2 · Use of lithium (Li+) for the preparation of a composition for transfection of a polynucleotide into a cell and compositions useful in gene therapy

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- Number: US 6,723,708 B2
- Document ID: US6723708B2
- Status: Granted
- Kind: B2
- Application number: 09/741,997
- Filed: 2000-12-22
- Published: 2001-08-30
- Granted: 2004-04-20
- Earliest claimed: 1999-12-12
- Assignees: Transgene S.A.
- Inventors: Serge Braun
- Web page: https://patentgrep.com/patent/US6723708B2

## CPC

- A61K 48/00: Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
- A61P 43/00: Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
- C12N 15/87: Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

Described is the use of lithium (Li+) for the preparation of a therapeutic composition for the introduction of a polynucleotide into a cell.

## Claims

### Claim 1

A method for improving the transfer of a polynucleotide into a cell comprising contacting a cell with 0.1 to about 10 mM of lithium chloride to improve the transfer of a polynucleotide into the cell.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the cells are simultaneously or subsequently contacted with polynucleotide.

### Claim 3 (depends on 1)

The method according to claim 1, wherein said tissue cell is muscle.

### Claim 4 (depends on 1)

The method according to claim 1, wherein the administration of lithium chloride is performed independently from a second administration of a composition containing at least one polynucleotide to the same cell.

### Claim 5 (depends on 4)

The method according to claim 4, wherein the administration of lithium chloride is performed prior to said second administration.

### Claim 6 (depends on 4)

The method according to claim 4, wherein the polynucleotide contains a gene and in capable of functionally expressing said gene in said cell.

### Claim 7 (depends on 4)

The method according to claim 4, wherein said polynucleotide is naked nucleic acid.

### Claim 8 (depends on 4)

The method according to claim 4, wherein said polynucleotide is a non-naked nucleic acid.

### Claim 9 (depends on 4)

The method according to claim 4, wherein the polynucleotide is administered alone with a pharmaceutically acceptable amount and is present in a concentration from about 0.01 mM to about 1 mM.

### Claim 10 (depends on 4)

The method according to claim 4, wherein said cells are contacted with lithium chloride before, simultaneously and subsequently to contact with the polynucleotide.

### Claim 11 (depends on 10)

The method according to claim 10, wherein the cells are simultaneously contacted with the lithium chloride and the polynucleotide.