Methods for determining toxicity reversing agents

A method for ameliorating toxicity in a patient undergoing therapy with a compound that has a known toxicity comprising:

• a) isolating lymphocytes from said patient;
• b) incubating lymphocytes in the presence of the compound and in the presence and absence of a candidate substance that can reverse the toxicity of said compound;
• c) comparing the toxic-effect of the compound on the lymphocytes incubated in the presence of the candidate substance to the effect of the compound on the lymphocytes in the absence of the candidate substance thereby identifying a substance that can reverse toxicity. wherein the toxic-effect of the compound is assessed by assessing its effect on the amount of ATP synthesized by the lymphocytes;
• and
• d) administrating to said patient the identified substance that can reverse toxicity of the compound.

A composition comprising one or more factors characterized by the following properties:

• a) isolatable from mammalian plasma;
• b) containing at least one heat-labile component having a molecular weight of at least 100,000;
• and
• c) having the ability to ameliorate the toxicity of a metabolic inhibitor.