# US 6,720,487 B1 · Inbred maize line PH4GP

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- Number: US 6,720,487 B1
- Document ID: US6720487B1
- Status: Granted
- Kind: B1
- Application number: 09/758,713
- Filed: 2001-01-11
- Granted: 2004-04-13
- Earliest claimed: 2001-01-11
- Assignees: Pioneer Hi-Bred International, Inc.
- Inventors: Loren John Hoffbeck
- Web page: https://patentgrep.com/patent/US6720487B1

## CPC

- A01H 6/4684: {Zea mays [maize]}
- A01H 5/10: Seeds
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES

## Abstract

An inbred maize line, designated PH4GP, the plants and seeds of inbred maize line PH4GP, methods for producing a maize plant, either inbred or hybrid, produced by crossing the inbred maize line PH4GP with itself or with another maize plant, and hybrid maize seeds and plants produced by crossing the inbred line PH4GP with another maize line or plant and to methods for producing a maize plant containing in its genetic material one or more transgenes and to the transgenic maize plants produced by that method. This invention also relates to inbred maize lines derived from inbred maize line PH4GP, to methods for producing other inbred maize lines derived from inbred maize line PH4GP and to the inbred maize lines derived by the use of those methods.

## Claims

### Claim 1

Seed of maize inbred line designated PH4GP, representative seed of said line having been deposited under ATCC Accesion No. PTA-4430.

### Claim 2 (depends on 1)

A maize plant, or a part thereof, produced by growing the seed of claim 1.

### Claim 3 (depends on 2)

The maize plant of claim 2 wherein said plant has been detasseled.

### Claim 4 (depends on 2)

A tissue culture of regenerable cells produced from the plant of claim 2.

### Claim 5 (depends on 4)

Protoplast produced from the tissue culture of claim 4.

### Claim 6 (depends on 4)

The tissue culture of claim 4, wherein cells of the tissue culture are from a tissue selected from the group consisting of leaf, pollen, embryo, root, root tip, another, silk, flower, kernel, ear, cob, husk and stalk.

### Claim 7 (depends on 4)

A maize plant generated from the tissue culture of claim 4, said plant having all the morphological and physiological characteristics of inbred line PH4GP, representative seed of said line having been deposited under ATCC Accession No. PTA-4430.

### Claim 8 (depends on 2)

A method for producing an F1 hybrid maize seed, comprising crossing the plant of claim 2 with a different maize plant and harvesting the resultant F1 hybrid maize seed.

### Claim 9 (depends on 2)

A method of producing a male maize plant comprising transforming the maize plant of claim 2 with a nucleic acid molecule that confers male sterility.

### Claim 10 (depends on 9)

A male sterile maize plant produced by the method of claim 9.

### Claim 11 (depends on 2)

A method of producing an herbicide resistant maize plant comprising transforming the maize plant of claim 2 with a transgene that confers herbicide resistance.

### Claim 12 (depends on 11)

An herbicide resistant maize plant produced by the method of claim 11.

### Claim 13 (depends on 12)

The maize plant of claim 12, wherein the transgene confers resistance to an herbicide selected from the group consisting of: imidazolinone, sulfonylurea, glyphosate, glufosinate, L-phosphinothricin, triazine and benzonitrile.

### Claim 14 (depends on 2)

A method of producing an insect resistant maize plant comprising transforming the maize plant of claim 2 with a transgene that confers insect resistance.

### Claim 15 (depends on 14)

An insect resistant maize plant produced by the method of claim 14.

### Claim 16 (depends on 15)

The maize plant of claim 15, wherein the transgene encodes a Bacillus thuringiensis endotoxin.

### Claim 17 (depends on 2)

A method of producing a disease resistant maize plant comprising transforming the maize plant of claim 2 with a transgene that confers disease resistant.

### Claim 18 (depends on 17)

A disease resistant maize plant produced by the method of claim 17.

### Claim 19 (depends on 2)

A method of producing a maize plant with decreased phytate content comprising transforming the maize plant of claim 2 with a transgene encoding phytase.

### Claim 20 (depends on 19)

A maize plant with decrease phytate content produced by the method of claim 19.

### Claim 21 (depends on 2)

A method of producing a maize plant with modified fatty acid metabolism or modified carbohydrate metabolism comprising transforming the maize plant of claim 2 with a transgene encoding a protein selected from the group consisting of stearyl-ACP desaturase, frutosyltransferase, levansucrase, alpha-amylase, in and starch branching enzyme.

### Claim 22 (depends on 21)

A maize plant produced the method of claim 21.

### Claim 23 (depends on 22)

The maize plant of claim 22 wherein the transgene confers a trait selected from the group consisting of waxy starch and increased amylose starch.

### Claim 24

A maize plant or part thereof, having all the physiological and morphological characteristics of the inbred line PH4GP, representative seed of said line having been deposited under ATCC Accession No. PTA-4430.

### Claim 25

A method of introducing a desired trait into maize inbred line PH4GP comprising:
(a) crossing PH4GP plant grown from PH4GP seed, representative seed of which has been deposited under ATCC Accession No. PTA-4430, with plants of another maize line that comprise a desired trait to produce F1 progeny plants, wherein that desired trait is selected from the group consisting of male sterility, herbicide resistance, insect resistance, disease resistance and waxy starch;
(b) selecting F1 progeny plants that have the desired trait to produce selected F1 progeny plants;
(c) crossing the selected progeny plants with the PH4GP plants to produce backcross progeny plants;
(d) selecting for backcross progeny plants that have the desired trait and physiological and morphological characteristics of maize inbred line PH4GP listed in Table 1 to produce selected backcross progeny plants; and
(c) repeating steps (c) and (d) three or more times in succession to produce selected fourth or higher backcross progeny plants that comprise the desired trait and all of the physiological and morphological characteristics of maize inbred line PH4GP listed in Table 1 as determined at the 5% significance level when grown in the same environmental conditions.

### Claim 26 (depends on 25)

A plant produced by the method of claim 25, wherein the plant has the desired trait and all of the physiological and morphological characteristics of maize inbred line PH4GP listed in Table 1 as determined at the 5% significance level when grown in the same environmental conditions.

### Claim 27 (depends on 26)

The plant of claim 26 wherein the desired trait is herbicide resistance and the resistance is conferred to an herbicide selected from the group consisting of: imidazolinone, sulfonylurea, glyphosate, glufosinate, L-phosphinothricin, triazine and benzonitrile.

### Claim 28 (depends on 26)

The plant of claim 26 wherein the desired trait is insect resistance and the insect resistance is conferred by a transgene encoding a Bacillis thuringiensis endotoxin.

### Claim 29 (depends on 26)

The plant of claim 26 wherein the desired trait is male sterility and the trait is conferred by a cytoplasmic nucleic acid molecule that confers male sterility.

### Claim 30

A method of modifying fatty acid metabolism, phytic acid metabolism or carbohydrate metabolism maize inbred line PH4GP comprising:
(a) crossing PH4GP plants grows from PH4GP seed, representative seed of which has been deposited under ATCC Accession No. PTA-4430, with plants of another maize line that comprise a nucleic acid molecule encoding an enzyme selected from the group consisting of phytase, stearyl-ACP desaturase, fructosyltansferance, levansucrase, alpha-amylase, invertase and starch branching enzyme;
(b) selecting F1 progeny plants that have said nucleic acid molecule to produce selected F1 progeny plants;
(c) Crossing the selected progeny plants with the PH4GP plants to produce backcross progeny plants;
(d) selecting for backcross progeny plants that have said nucleic acid molecule and physiological and morphological characteristics of maize inbred line PH4GP listed in Table 1 to produce selected backcross progeny plants; and
(e) repeating steps (c) and (d) three or more times in succession to produce selected fourth or higher backcross progeny plants that comprise said nucleic acid molecule and have all of the physiological and morphological characteristics of maize inbred line PH4GP listed in Table 1 as determined at the 5% significance level when grown in the same environmental conditions.

### Claim 31 (depends on 30)

A plant produced by the method of claim 30, wherein the plant comprises the nucleic acid molecule and has all of the physiological and morphological characteristics of maize inbred line PH4GP listed in Table 1 as determined at the 5% significance level when grown in the same environmental conditions.