# US 6,720,477 B2 · Signal transduction stress-related proteins and methods of use in plants > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 6,720,477 B2 - Document ID: US6720477B2 - Status: Granted - Kind: B2 - Application number: 09/828,447 - Filed: 2001-04-06 - Published: 2002-06-06 - Granted: 2004-04-13 - Earliest claimed: 2000-04-07 - Assignees: BASF Plant Science GmbH - Inventors: Oswaldo da Costa e Silva; Hans J. Bohnert; Nocha van Thielen; Ruoying Chen; Manabu Ishitani - Web page: https://patentgrep.com/patent/US6720477B2 ## CPC - C12N 15/8273: {for drought, cold, salt resistance} - C07K 14/415: from plants - C12N 9/1205: {Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases} - C12N 9/16: acting on ester bonds (3.1) - C12N 15/8217: {Gene switch} - A01K 2217/05: Animals comprising random inserted nucleic acids (transgenic) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - A01K: ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS ## Abstract A transgenic plant transformed by a Signal Transduction Stress-Related Protein (STSRP) coding nucleic acid, wherein expression of the nucleic acid sequence in the plant results in increased tolerance to environmental stress as compared to a wild type variety of the plant. Also provided are agricultural products, including seeds, produced by the transgenic plants. Also provided are isolated STSRPs, and isolated nucleic acid coding STSRPs, and vectors and host cells containing the latter. ## Claims ### Claim 1 A transgenic plant cell transformed with a nucleic acid encoding a polypeptide, wherein the polypeptide is defined in SEQ ID NO:12. ### Claim 2 (depends on 1) The transgenic plant cell of claim 1, wherein the nucleic acid comprises the polynucleotide as defined in SEQ ID NO:7. ### Claim 3 A transgenic plant cell transformed with a nucleic acid encoding a polypeptide wherein expression of the polypeptide in the plant cell results in the plant cell's increased tolerance to an environmental stress selected from one or more of the group consisting of drought and temperature less than or equal to 0° C. as compared to an untransformed wild type variety of the plant cell; wherein the nucleic acid hybridizes under stringent conditions to at least one sequence selected from the group consisting of the sequence of SEQ ID NO:7 and the full-length complement of the sequence of SEQ ID NO:7; and wherein the stringent conditions comprise hybridization in a 6× sodium chloride/sodium citrate (SSC) solution at 65° C. and at least one wash in a 0.2× SCC, 0.1% SDS solution at 50° C. ### Claim 4 A transgenic plant cell transformed with a nucleic acid encoding a polypeptide having at least 90% sequence identity with a polypeptide as defined in SEQ ID NO:12, wherein expression of the polypeptide in the plant cell results in the plant cell's increased tolerance to an environmental stress selected from one or more of the group consisting of drought and temperature less than or equal to 0° C. as compared to an untransformed wild type variety of the plant cell. ### Claim 5 (depends on 1, 2, 3) The transgenic plant cell of any one of claims 1, 2, 3, or 4, wherein the plant is a monocot. ### Claim 6 (depends on 1, 2, 3) The transgenic plant cell of any one of claims 1, 2, 3, or 4, wherein the plant is a dicot. ### Claim 7 (depends on 1, 2, 3) The transgenic plant cell of any one of claims 1, 2, 3, or 4, wherein the plant is selected from the group consisting of maize, wheat, rye, oat, triticale, rice, barley, soybean, peanut, cotton, rapeseed, canola, manihot, pepper, sunflower, tagetes, solanaceous plants, potato, tobacco, eggplant, tomato, Vicia species, pea, alfalfa, coffee, cacao, tea, Salix species, oil palm, coconut, and perennial grass. ### Claim 8 (depends on 1, 2, 3) A transgenic plant comprising the plant cell according to any one of claims 1, 2, 3, or 4. ### Claim 9 (depends on 1, 2, 3) A seed produced by a transgenic plant comprising the plant cell according to any one of claims 1, 2, 3, or 4, wherein the seed comprises the nucleic acid encoding the polypeptide, wherein the seed is true breeding for an increased tolerance to an environmental stress as compared to an untransformed wild type variety of the plant cell, and wherein the environmental stress is selected from one or more of the group consisting of drought and low temperature less than or equal to 0° C. ### Claim 10 An isolated nucleic acid encoding a polypeptide, wherein the nucleic acid comprises a polynucleotide that encodes the polypeptide as defined in SEQ ID NO:12. ### Claim 11 (depends on 10) The isolated nucleic acid of claim 10, wherein the nucleic acid comprises the polynucleotide as defined in SEQ ID NO:7. ### Claim 12 An isolated nucleic acid encoding a polypeptide, wherein expression of the polypeptide in the plant cell results in the plant cell's increased tolerance to an environmental stress selected from one or more of the group consisting of drought and temperature less than or equal to 0° C. as compared to an untransformed wild woe variety of the plant cell; wherein the nucleic acid hybridizes under stringent conditions to at least one sequence selected from the group consisting of the sequence of SEQ ID NO:7 and the full-length complement of the sequence of SEQ ID NO:7; and wherein the stringent conditions comprise hybridization in a 6× sodium chloride/sodium citrate (SSC) solution at 65° C. and at least one wash in a 0.2× SSC, 0.1% SDS solution at 50° C. ### Claim 13 An isolated nucleic acid, encoding a polypeptide having at least 90% sequence identity with the polypeptide as defined in SEQ ID NO:12, wherein expression of the polypeptide in the plant cell results in the plant cell's increased tolerance to an environmental stress selected from one or more of the group consisting of drought and temperature less than or equal to 0° C. as compared to an untransformed wild type variety of the plant cell. ### Claim 14 (depends on 10, 11, 12) An isolated recombinant expression vector comprising the nucleic acid of any one of claims 10, 11, 12, or 13, wherein expression of the polypeptide in a plant cell results in the plant cell's increased tolerance to an environmental stress, as compared to an untransformed wild type variety of the plant cell, and wherein the environmental stress is selected from one or more of the group consisting of drought and temperature less than or equal to 0° C. ### Claim 15 (depends on 14) A method of producing a transgenic plant comprising a nucleic acid encoding a polypeptide, comprising, a. transforming a plant cell with the expression vector of claim 14, and b. generating from the plant cell a transgenic plant that expresses the polypeptide; wherein the polypeptide no polypeptide is defined in SEQ ID NO:12. ### Claim 16 (depends on 15) The method of claim 15, wherein the expression vector comprises the polynucleotide as defined in SEQ ID NO:7. ### Claim 17 (depends on 14) A method of producing a transgenic plant comprising a nucleic acid encoding a polypeptide, wherein expression of the polypeptide in the plant results in the plant's increased tolerance to an environmental stress, as compared to a an untransformed wild type variety of the plant, comprising, a. transforming a plant cell with the expression vector of claim 14; and b. generating from the plant cell a transgenic plant that expresses the polypeptide; wherein the nucleic acid hybridizes under stringent conditions to at least one sequence selected from the group consisting of the sequence of SEQ ID NO:7 and the full-length complement of the sequence of SEQ ID NO:7; wherein the stringent conditions comprise hybridization in a 6× sodium chloride/sodium citrate (SSC) solution at 65° C. and at least one wash in a 0.2× SSC, 0.1% SDS solution at 50° C.; and wherein the environmental stress is selected from one or more of the group consisting of drought and low temperature less than or equal to 0° C. ### Claim 18 (depends on 14) A method of producing a transgenic plant comprising a nucleic acid encoding a polypeptide, wherein expression of the polypeptide in the plant results in the plant's increased tolerance to an environmental stress, as compared to an untransformed wild type variety of the plant, comprising, a. transforming a plant cell with the expression vector of claim 14; and b. generating from the plant that expresses the polypeptide; wherein the polypeptide has at least 90% sequence identity with the polypeptide as defined in SEQ ID NO:12, and wherein the environmental stress is selected from one or more of the group consisting of drought and temperature less than or equal to 0° C.