Nucleic acid sequence encoding FLP recombinase
AssigneePioneer Hi-Bred International, Inc.
InventorsChristopher L. Baszczynski, Benjamin A. Bowen, Bruce J. Drummond, William J. Gordon-Kamm, David J. Peterson, Gary A. Sandahl, Laura A. Tagliani, Zuo-Yu Zhao, Grace marie St. Clair
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Abstract
A nucleic acid sequence effectively expressing FLP recombinase in monocot plants, particularly in maize. Stable, transformed maize plants harboring a gene encoding FLP or harboring FRT nucleic acid sequences enable efficient site-directed recombination of nucleic acid sequences in a monocot's genome.
Claims27
An isolated nucleotide sequence encoding an FLP recombinase wherein said sequence comprises at least 80% monocot-preferred codons, wherein said monocot-preferred codons are those codons that have a frequency of codon usage in a monocot plant of greater than 20%.
A method for excising or inverting a target nucleotide sequence in a plant cell, said method comprising introducing a nucleotide sequence encoding an FLP recombinase wherein said nucleotide sequence encoding an FLP recombinase comprises at least 80% monocot-preferred codons, wherein said monocot-preferred codons are those codons that have a frequency of codon usage in a monocot plant of greater than 20%, and wherein said target sequence is flanked by FRT sites.
A transformed plant containing within its genome a nucleotide sequence encoding an FLP recombinase wherein said sequence comprises at least 80% monocot-preferred codons, wherein said monocot-preferred codons are those codons that have a frequency of codon usage in a monocot plant of greater than 20%.
An isolated nucleotide sequence encoding an FLP recombinase wherein said sequence comprises at least 80% maize-preferred codons, wherein said maize-preferred codons are those codons that have a frequency of codon usage in a maize plant of greater than 20%.
A method for excising or inverting a target nucleotide sequence in a plant cell, said method comprising introducing a nucleotide sequence encoding an FLP recombinase wherein said nucleotide sequence encoding an FLP recombinase comprises at least 80% maize-preferred codons, wherein said maize-preferred codons are those codons that have a frequency of codon usage in a maize plant of greater than 20%, wherein said target sequence is flanked by FRT sites.
A transformed plant containing within its genome a nucleotide sequence encoding an FLP recombinase wherein said sequence comprises at least 80% maize-preferred codons, wherein said maize-preferred codons are those codons that have a frequency of codon usage in a maize plant of greater than 20%.
Dependencies are inferred from the claim text.