# US 6,720,473 B1 · Intra-vascular administration of particles to induce pulmonary hypertension, pulmonary hypertension syndrome, and ascites in poultry

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- Number: US 6,720,473 B1
- Document ID: US6720473B1
- Status: Granted
- Kind: B1
- Application number: 09/913,774
- Filed: 2001-08-13
- Granted: 2004-04-13
- Earliest claimed: 2000-01-14
- Assignees: University of Arkansas
- Inventors: Gisela F. Erf; Robert F. Wideman, Jr.; Howard L. French
- Web page: https://patentgrep.com/patent/US6720473B1

## CPC

- A01K 67/027: New or modified breeds of vertebrates
- A61B 5/412: {Detecting or monitoring sepsis}
- A61B 5/415: {the glands, e.g. tonsils, adenoids or thymus}
- A61B 5/416: {the spleen}
- A61B 5/418: {lymph vessels, ducts or nodes}
- A01K 2227/30: Bird
- A01K 2267/0375: Animal model for cardiovascular diseases
- A61B 5/021: Measuring pressure in heart or blood vessels
- A01K: ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D)

## Abstract

A new method for identifying and eliminating chickens that are susceptible to pulmonary hypertension uses micrometer scale particles to occlude blood vessels in the lungs of the chickens. This results in the death of chickens that are pre-disposed to pulmonary hypertension. The invention effectively culls a chicken stock of the weaker animals.

## Claims

### Claim 1

A method of producing improved genetic breeding stocks in chickens, the method comprising:
a) inducing pulmonary hypertension in chickens by occluding the pulmonary vasculature with particulate substances of a size about 8 to about 250 micrometers in diameter injected into veneous blood that carries the particles to the right cardiac ventricle pumping the blood containing the particles to the lungs, wherein the particles directly increase pulmonary vascular resistance by lodging in small blood vessels, the heart being forced to develop and maintain an elevated pressure in the pulmonary arteries to propel the reguisite cardiac output through vessels remaining unoccluded, chickens that are susceptible to pulmonary hypertension subsequently developing pulmonary hypertension syndrome, and
b) separating susceptible chickens from the resistant chickens, the resistant chickens being useful as genetic breeding stock resistant to pulmonary hypertension.

### Claim 2

A method for producing a pulmonary hypertension resistant poultry strain comprising:
a) administering into the pulmonary circulation of immature poultry, particles having about 8 to about 250 μM diameter in an amount sufficient to kill or disable poultry susceptible to pulmonary hypertension while resistant poultry remain unaffected; and
b) selecting and breeding the resistant poultry to produce a pulmonary hypertension resistant poultry strain.