# US 6,720,419 B2 · Sulfated fucan oligosaccharide

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- Number: US 6,720,419 B2
- Document ID: US6720419B2
- Status: Granted
- Kind: B2
- Application number: 10/278,942
- Filed: 2002-10-24
- Published: 2003-09-11
- Granted: 2004-04-13
- Earliest claimed: 2001-10-24
- Assignees: Takara Bio Inc.
- Inventors: Takeshi Sakai; Hitomi Amarume; Takashi Kawai; Kaoru Kojima; Kazuo Shimanaka; Katsushige Ikai; Ikunoshin Kato
- Web page: https://patentgrep.com/patent/US6720419B2

## CPC

- C08B 37/006: {Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof}
- C08B: POLYSACCHARIDES; DERIVATIVES THEREOF (polysaccharides containing less than six saccharide radicals attached to each other by glycosidic linkages C07H; fermentation or enzyme-using processes C12P19/00; sugar industry C13; production of cellulose D21)

## Abstract

A smaller molecule obtainable by allowing a sulfated fucan-digesting enzyme which digests a novel sulfated polysaccharide derived from an alga belonging to Laminariales to act on a sulfated fucan, and a method for producing the same.

## Claims

### Claim 1

A sulfated fucan having the following chemical and physical properties:
(1) containing fucose as a constituting saccharide;
(2) containing a sulfated saccharide of general formula (I) as an essential component of the constituting saccharide:
wherein R is H or SO3H, at least one of Rs is SO3H and n is an integer of 1 or more; and
(3) being converted into smaller molecules by a sulfated fucan-digesting enzyme derived from Alteromonas sp. SN-1009 to generate at least one compound selected from the group consisting of the compounds of general formulas (II), (III), (XIII), (XIV), (XV) and (XVI):
wherein R is H or SO3H and at least one of Rs is SO3H in all formulas above,
or a salt thereof.

### Claim 2

A sulfated fucan oligosaccharide of general formula (I):
wherein R is H or SO3H, at least one of Rs is SO3H and n is 1 to 5.

### Claim 3 (depends on 1, 2)

A method for preparing a sulfated fucan oligosaccharide, the method comprising:
allowing a sulfated fucan-digesting enzyme derived from Alteromonas sp. SN-1009 to act on a sulfated fucan defined by claim 1 or 2; and
collecting a digestion product.

### Claim 4 (depends on 3)

The method according to claim 3, wherein the sulfated fucan is derived from Kjellmaniella crassifolia, Laminaria japonica or Lessonia nigrescens.

### Claim 5

A sulfated fucan oligosaccharide having a chemical structure selected from the group consisting of general formulas (II), (III), (XIII), (XIV), (XV) and (XVI):
wherein R is H or SO3H and at least one of Rs is SO3H in all formulas above, or a salt thereof.