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AssigneeNanosphere, Inc.
InventorsChad A. Mirkin, Robert L. Letsinger, Robert C. Mucic, James J. Storhoff, Robert Elghanian, Thomas A. Taton
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The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.
A preassembled core probe for detecting the presence or absence of a target nucleic acid bound to a substrate, the core probe comprising at least two types of nanoparticles having oligonuleotides attached thereto, the nonoparticles of the core probe being bound to each other as a result of the hybridization of a portion of the oligonucleotides attached to them, and another portion of the oligonucleotides bound to at least one type of nanoparticles have a sequence that is complementary to at least one portion of a sequence of the target nucleic acid.
A kit for detecting the presence or absence of a target nucleic acid bound to a substrate, the target nucleic acid having a sequence of at least two portions, the kit comprising at least one container holding a preassembled core probe comprising at least two types of nanoparticles having oligonucleotides attached thereto, the nanoparticles of the core probe being bound to each other as a result of the hybridization of a portion of the oligonucleoties attached to them, and another portion of the oligonucleotides bound to at least one type of nanoparticles have a sequence that is complementary to at least a portion of a sequence of the target nucleic acid.
A kit for detecting the presence or absence of a target nucleic acid bound to a substrate, the target nucleic acid having a sequence of at least two portions, the kit (i) at least one container holding a preassembled core probe comprising at least two types of nanoparticles having oligonucleotides attached thereto, the nanoparticles of the core probe being bound to each other as a result of the hybridization of a portion of the oligonucleotides attached to them;
A kit for detecting the presence or absence of a target nucleic acid bound to a substrate, the target nucleic acid having a sequence of at least two portions, the kit (i) at least one container holding a preassembled core probe comprising at least two types of nanoparticles having oligonucleotides attached thereto, the nanoparticles of the core probe being bound to each other as a result of the hybridization of a portion of the oligonucleotides attached to them;
Dependencies are inferred from the claim text.