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US 6,720,310 B1Granted
Transfer method for specific cellular localization of nucleic acids
AssigneeAvaris AB
InventorsLars Branden, Abdalla J. Mohamed, C. I. Edvard Smith
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AssigneeAvaris AB
InventorsLars Branden, Abdalla J. Mohamed, C. I. Edvard Smith
Estimating the term…
The present invention relates to a novel method of genetic modification, wherein a nucleic acid of interest is transferred across a biological membrane, and/or directed to a specific location within or on a cell, by use of a synthetic transport entity. The transport entity according to the invention is new as such and produced by coupling a functional element (FE), such as a nuclear localization signal (NLS), an antennapedia peptide of a protein comprising both membrane translocation and nuclear transport properties, to a binding element (BE), such as a peptide nucleic acid (PNA), preferably separated by a linker molecule, which combination is then hybridized to a BE target sequence present on a carrier, which also includes the nucleic acid of interest. The present nucleic acid of interest may for example be a gene encoding a peptide, a protein or an RNA, or any other nucleic acid useful in genetic recombination events.
A synthetic transport complex for transferring a nucleic acid of interest across a biological membrane into a cell, wherein the complex is comprised of two or more functional elements (FE), each of which is complexed to a binding element (BE) in the form of a peptide nucleic acid (PNA), and a nucleic acid carrier, which comprises at least two BE target sequences and a nucleic acid of interest in a vector;
A kit comprising components for making a transport entity capable of transferring a nucleic acid of interest across a biological membrane into a cell, which kit comprises at least two binding elements (BE) in the form of a peptide nucleic acid (PNA);
Dependencies are inferred from the claim text.