# US 6,720,180 B2 · Wounded epithelium-specific transcript

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- Number: US 6,720,180 B2
- Document ID: US6720180B2
- Status: Granted
- Kind: B2
- Application number: 09/859,961
- Filed: 2001-05-17
- Published: 2003-09-04
- Granted: 2004-04-13
- Earliest claimed: 2001-02-15
- Assignees: Board of Regents, The University of Texas System
- Inventors: Brian K. Pilcher
- Web page: https://patentgrep.com/patent/US6720180B2

## CPC

- C07K 14/47: from mammals
- A01K 2217/05: Animals comprising random inserted nucleic acids (transgenic)
- A01K 2217/075: inducing loss of function, i.e. knock out
- A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00)
- A61K 48/00: Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A01K: ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

The instant invention discloses compositions and methods related to epsin 3, a novel protein closely related to, but distinct from previously described epsins. Epsin 3 mRNA and protein are undetectable in keratinocytes isolated from unwounded skin, but is induced in cells following contact with fibrillar type I collagen. It is envisioned that this protein plays an important role in activated epithelial cells during tissue morphogenesis.

## Claims

### Claim 1

An isolated and purified polynucleotide comprising a nucleic acid sequence encoding polypeptide of SEQ ID NO:2.

### Claim 2 (depends on 1)

The polynucleotide of claim 1, comprising the nucleic acid sequence set forth in SEQ ID NO:1.

### Claim 3

An expression vector comprising a promoter operably linked to a nucleic acid sequence that encodes SEQ ID NO: 2.

### Claim 4 (depends on 3)

The expression vector of claim 3, wherein the expression vector is a viral vector.

### Claim 5 (depends on 3)

The expression vector of claim 3, wherein the nucleic acid sequence comprises the nucleic acid sequence of SEQ ID NO:1.

### Claim 6 (depends on 3)

The expression vector of claim 3, wherein the expression vector is a nonviral vector.

### Claim 7 (depends on 6)

The expression vector of claim 6, wherein the vector is comprised in a liposome.

### Claim 8 (depends on 1)

A vector comprising the polynucleotide of claim 1.

### Claim 9 (depends on 2)

A vector comprising the polynucleotide of claim 2.