Method for determining alkaline phosphatase and eliminating haemoglobin disturbances
AssigneeRoche Diagnostics GmbH
InventorsRalph Weisheit, Wolfgang Treiber
Life of this patent
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Abstract
The invention concerns a method for the determination of alkaline phosphatase in a sample by optical measurement in which interference by free haemoglobin or blood substitutes is eliminated by means of certain wavelength combinations, a method for eliminating interference caused by free haemoglobin or blood substitutes in a determination of alkaline phosphatase and the use of certain wavelength combinations to eliminate interference by free haemoglobin or blood substitutes.
Claims21
A method for determining alkaline phosphatase in a sample in which interference by hemoglobin is eliminated, comprising:
- adding 4-nitrophenyl phosphate to said sample;
- simultaneously determining a first optical measurement of said sample at 450±10 nm and a second optical measurement at one or more secondary wavelengths selected from the group consisting of 480±10 nm, 546±10 nm, and 575±10 nm;
- and
- determinining the difference between the first and second optical measurements.
A method for determining a level of alkaline phosphatase in a sample containing 4-nitrophenyl phosphate in which interference by hemoglobin is eliminated, the method comprising:
- simultaneously determining a first optical measurement at 450±10 nm and a second optical measurement at a secondary wavelength selected from the group consisting of 480±10 nm, 546±10 nm, and 575±10 nm;
- and
- determining the difference between the first and second optical measurements.
A method for determining a level of alkaline phosphatase in a sample in which interference by hemoglobin is eliminated, comprising:
- adding 4-nitrophenyl phosphate to said sample;
- simultaneously measuring a first change in absorbance of said sample at 450±10 nm and
- a second change in absorbance of said sample at 480±10 nm, 546±10 nm, or 575±10 nm;
- and
- determining the difference between the first and second changes in absorbance.
Dependencies are inferred from the claim text.