Method for simultaneous detection of multiple microbial antigens in biological specimens from mastitic animals
AssigneeHelica BioSystems, Inc.
InventorWondu Wolde-Mariam
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Abstract
The present invention involves a method and an immuno-analytical device for the rapid and simultaneous detection of multiple micro-organisms in the biological fluids from milk-producing animals suffering from mastitis. This method is based on a lateral flow immuno-assay technique performed to detect antigens specific for multiple infectious agents which are known to cause and/or be encountered in cases of mastitis. Mastitis is an inflammatory condition affecting the udders of milk-producing animals as a result of microbial infections.
Claims23
A method for simultaneously detecting a plurality of different indicators of mastitis in a milk sample of a mammal, comprising the steps of:
- exposing said milk sample to a test membrane having antibodies that specifically bind to a plurality of different pathogens selected from the group consisting of a Streptococcus species, a Staphylococcus species, and a Mycoplasma species;
- said test membrane further having antibodies that specifically bind to granulocytes in said milk sample;
- detecting the presence or absence of the pathogens and the amount of granulocytes in said milk sample;
- and
- relating the presence of said pathogens and said granulocytes in said milk sample to mastitis in said mammal.
Dependencies are inferred from the claim text.