Microsatellite markers for plants of the species Triticum aestivum and Tribe triticeae and the use of said markers
AssigneeInstitut fur Pflanzengenetik und Kulturpflanzenforschung
InventorsMarion Roder, Jens Plaschke, Martin Ganal
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Abstract
A set of a plurality of microsatellite markers is provided for plant species of the tribe Triticeae, particularly Triticum aestivum. Each of the markers are constituted by a sequence tagged site (STS) defined by two primers, specific to a particular microsatellite sequence. Each primer has an average length of 20±3 bases and flanks the particular microsatellite sequence. Each of the microsatellite markers is formed by amplification of the microsatellite sequence by a polymerase chain reaction, to form markers of different length.
Claims3
A method of genotyping plants of the Triticum aestivum species and the genus Triticeae at a microsatellite locus, the method comprising
- a) amplifying chromosomal DNA with one or more oligonucleotide primer pairs specifically hybridizing to said locus of a region of said chromosomal DNA, wherein said region of the DNA comprises a repeated dinucleotide motif comprising at least one of the following selected from the group consisting of (GA:CT)n, (GT:CA)n, (AT:TA)n, where n≧10, to obtain an amplification product,
- b) wherein each primer pair consists of a first oligonucleotide of SEQ ID NO. x and a second oligonucleotide of SEQ 10 NO. x+1 , and wherein x=1, 3, 5, 7, 9, 11, 13, 15, 17, 19;
- and
- c) size fractionating the amplification product to provide a measure of the said motif of the chromosomal DNA between said primer pairs,
- wherein the size of the amplification product is polymorphic for said locus and provides a marker for genotyping said plants.
Dependencies are inferred from the claim text.